Abstract
Breast cancer occurring during pregnancy (PrBC) and postpartum (PPBC) is usually diagnosed at more advanced stages compared with other breast cancer, worsening its prognosis. PPBC is particularly aggressive, with increased metastatic risk and mortality. Thus, effective screening methods to detect early PrBC and PPBC are needed. We report for the first time that cell-free tumor DNA (ctDNA) is present in breast milk (BM) collected from patients with breast cancer. Analysis of ctDNA from BM detects tumor variants in 87% of the cases by droplet digital PCR, while variants remain undetected in 92% of matched plasma samples. Retrospective next-generation sequencing analysis in BM ctDNA recapitulates tumor variants, with an overall clinical sensitivity of 71.4% and specificity of 100%. In two cases, ctDNA was detectable in BM collected 18 and 6 months prior to standard diagnosis. Our results open up the potential use of BM as a new source for liquid biopsy for PPBC detection.
Significance:
For the first time, we show that BM obtained from patients with breast cancer carries ctDNA, surpassing plasma-based liquid biopsy for detection and molecular profiling of early-stage breast cancer, even prior to diagnosis by image.
Keywords
Breast cancer; Breast milk
Bibliographic citation
Saura C, Ortiz C, Matito J, Arenas EJ, Suñol A, Martín Á, et al. Early-Stage Breast Cancer Detection in Breast Milk. Cancer Discov. 2023 Oct 1;13(10):2180–2191.
Audience
Professionals
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https://hdl.handle.net/11351/10430This item appears in following collections
- HVH - Articles científics [3000]
- VHIO - Articles científics [870]
- VHIR - Articles científics [1211]
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