dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Dopico, Eva |
dc.contributor.author | Pimenta del Rei, Rodrigo |
dc.contributor.author | Espinoza, Bertha |
dc.contributor.author | Ubillos, Itziar |
dc.contributor.author | Zanchin, Nilson Ivo Tonin |
dc.contributor.author | Sulleiro Igual, Elena |
dc.contributor.author | Moure Garcia, Zaira |
dc.date.accessioned | 2019-05-07T07:01:33Z |
dc.date.available | 2019-05-07T07:01:33Z |
dc.date.issued | 2019-03-12 |
dc.identifier.citation | Dopico E, Del-Rei RP, Espinoza B, Ubillos I, Zanchin NIT, Sulleiro E, et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infect Dis. 2019;19(1):251. |
dc.identifier.issn | 1471-2334 |
dc.identifier.uri | https://hdl.handle.net/11351/4056 |
dc.description | Chagas disease; Chimeric antigens; Trypanosoma cruzi |
dc.description.abstract | BACKGROUND:
Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease.
METHODS:
Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies.
RESULTS:
The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals.
CONCLUSIONS:
Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings. |
dc.language.iso | eng |
dc.publisher | BMC |
dc.relation.ispartofseries | BMC Infectious Diseases;19(1) |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.source | Scientia |
dc.subject | Antígens - Immunologia |
dc.subject | Chagas, Malaltia de - Barcelona |
dc.subject | Immigrants - Malalties - Diagnòstic |
dc.subject.mesh | Antigens, Protozoan |
dc.subject.mesh | Chagas Disease |
dc.subject.mesh | Emigrants and Immigrants |
dc.subject.mesh | /statistics & numerical data |
dc.subject.mesh | Barcelona |
dc.title | Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.1186/s12879-019-3872-z |
dc.subject.decs | antígenos protozoarios |
dc.subject.decs | enfermedad de Chagas |
dc.subject.decs | emigrantes e inmigrantes |
dc.subject.decs | /estadística & datos numéricos |
dc.subject.decs | Barcelona |
dc.relation.publishversion | https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-019-3872-z |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Dopico E, Ubillos I] Laboratori Clínic Territorial Metropolitana Sud, Institut Català de la Salut, Barcelona, Spain. [Del-Rei RP] Faculty of Technology and Sciences of Bahia, Salvador, Bahia, Brazil. [Espinoza B] Instituto de Investigaciones Biomédicas, Departamento de Inmunología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. [Zanchin NIT] Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil. [Sulleiro E, Moure Z] Servei de Microbiologia, Hospital Universitari Vall d'Hebron, Barcelona, Spain. |
dc.identifier.pmid | 30871504 |
dc.identifier.wos | WOS:000461371800002 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |