dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Román Canal, Berta |
dc.contributor.author | Moiola, Cristian Pablo |
dc.contributor.author | Gatius, Sònia |
dc.contributor.author | Bonnin, Sarah |
dc.contributor.author | Ruiz-Miró, Maria |
dc.contributor.author | González, Esperanza |
dc.contributor.author | Gil Moreno, Antonio |
dc.contributor.author | Colas Ortega, Eva |
dc.date.accessioned | 2019-08-07T06:27:20Z |
dc.date.available | 2019-08-07T06:27:20Z |
dc.date.issued | 2019-06-18 |
dc.identifier.citation | Roman-Canal B, Moiola CP, Gatius S, Bonnin S, Ruiz-Miró M, González E, et al. EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer. Cancers (Basel). 2019;11(6):e839. |
dc.identifier.issn | 2072-6694 |
dc.identifier.uri | https://hdl.handle.net/11351/4250 |
dc.description | Ascitic fluid; Biomarkers; Endometrial cancer |
dc.description.abstract | Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC. |
dc.language.iso | eng |
dc.publisher | MDPI |
dc.relation.ispartofseries | Cancers;11(6) |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | Endometri - Càncer - Diagnòstic |
dc.subject | MicroARN |
dc.subject | Marcadors tumorals |
dc.subject.mesh | Endometrial Neoplasms |
dc.subject.mesh | /diagnosis |
dc.subject.mesh | MicroRNAs |
dc.subject.mesh | Biomarkers, Tumor |
dc.title | EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.3390/cancers11060839 |
dc.subject.decs | neoplasias endometriales |
dc.subject.decs | /diagnóstico |
dc.subject.decs | microARN |
dc.subject.decs | marcadores tumorales |
dc.relation.publishversion | https://www.mdpi.com/2072-6694/11/6/839 |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Roman-Canal B] Departament de Patologia i Genètica Molecular, Grup de Patologia Oncològica, Institut de Recerca Biomèdica de Lleida, Lleida, Spain. Universitat de Lleida, Lleida, Spain. Departament de Patologia, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Spain. Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona, Spain. Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Moiola CP] Departament de Patologia i Genètica Molecular, Grup de Patologia Oncològica, Institut de Recerca Biomèdica de Lleida, Lleida, Spain. Universitat de Lleida, Lleida, Spain. Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain. [Gatius S, Ruiz-Miró M] Departament de Patologia i Genètica Molecular, Grup de Patologia Oncològica, Institut de Recerca Biomèdica de Lleida, Lleida, Spain. Universitat de Lleida, Lleida, Spain. [Bonnin S] Centre de Regulació Genòmica (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain. [González E] Exosomes Laboratory and Metabolomics Platform. CIC bioGUNE, CIBEREHD Bizkaia Technology Park, Derio, Spain. [Gil-Moreno A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. Servei de Ginecologia Oncològica, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Colas E] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain |
dc.identifier.pmid | 31216648 |
dc.identifier.wos | WOS:000475351200103 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |