dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Aznar Ruiz de Alegria, Maria Luisa |
dc.contributor.author | Moreno, María Milagros |
dc.contributor.author | Espasa Soley, Mateu |
dc.contributor.author | Sulleiro Igual, Elena |
dc.contributor.author | Bocanegra García, Maria Cristina |
dc.contributor.author | Gil Olivas, Eva |
dc.contributor.author | Escartin Huesca, Carlos |
dc.contributor.author | Vegue-Collado, Josep |
dc.contributor.author | Vivas-Cano, Maria-Carmen |
dc.contributor.author | Pumarola Suñé, Tomàs |
dc.contributor.author | Tórtola Fernández, Mª Teresa |
dc.contributor.author | Rando Segura, Ariadna |
dc.contributor.author | Molina Romero, Israel |
dc.date.accessioned | 2022-05-03T12:40:50Z |
dc.date.available | 2022-05-03T12:40:50Z |
dc.date.issued | 2021-10-12 |
dc.identifier.citation | Rando-Segura A, Aznar ML, Moreno MM, Espasa Soley M, Sulleiro Igual E, Bocanegra Garcia C, et al. Molecular characterization of rpoB gene mutations in isolates from tuberculosis patients in Cubal, Republic of Angola. BMC Infect Dis. 2021 Oct 12;21:1056. |
dc.identifier.issn | 1471-2334 |
dc.identifier.uri | https://hdl.handle.net/11351/7463 |
dc.description | Angola; Rifampicin; rpoB mutations |
dc.description.abstract | Background
The importance of Mycobacterium tuberculosis strains with disputed rpoB mutations remains to be defined. This study aimed to assess the frequency and types of rpoB mutations in M. tuberculosis isolates from Cubal, Angola, a country with a high incidence of tuberculosis.
Methods
All isolates included (n = 308) were analyzed using phenotypic drug susceptibility testing and GenoType MTBDRplus assay. DNA sequencing of the rpoB gene and determination of rifampicin MIC by macrodilution method were additionally performed on isolates yielding discordant results (n = 12) and those in which the mutation detected was not characterized (n = 8).
Results
In total, 85.1% (74/87) of rifampicin-resistant strains had undisputed rpoB mutations -S450L (49), D435V (15), H445D (3), H445Y (2), Q432ins (1), L449M plus S450F (1), S450F (1), S450W (1) and S450Y (1)-; 10.3% (9/87) had disputed rpoB mutations—L430P plus S493L (1), N437del (1), H445L (3), D435Y (2), L452P (2)-, 2.3% (2.3%) showed no rpoB mutations and 2.3% (2/87) showed heteroresistance—D435Y plus L452P and L430P plus S493L-.
Conclusion
Disputed rpoB mutations were common, occurring in 10.3% of rifampicin resistant isolates. Current phenotyping techniques may be unable to detect this resistance pattern. To increase their sensitivity, a lower concentration of RIF could be used in these tests or alternatively, rpoB mutations could be screened and characterized in all M. tuberculosis strains. |
dc.language.iso | eng |
dc.publisher | BMC |
dc.relation.ispartofseries | BMC Infectious Diseases;21 |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | Tuberculosi - Epidemiologia |
dc.subject | RNA-polimerases |
dc.subject | Tests de sensibilitat microbiològica |
dc.subject.mesh | Tuberculosis |
dc.subject.mesh | DNA-Directed RNA Polymerases |
dc.subject.mesh | Microbial Sensitivity Tests |
dc.title | Molecular characterization of rpoB gene mutations in isolates from tuberculosis patients in Cubal, Republic of Angola |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.1186/s12879-021-06763-8 |
dc.subject.decs | tuberculosis |
dc.subject.decs | ARN polimerasas dirigidas por ADN |
dc.subject.decs | pruebas de sensibilidad microbiana |
dc.relation.publishversion | https://doi.org/10.1186/s12879-021-06763-8 |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Rando-Segura A, Espasa Soley M, Sulleiro Igual E, Escartin Huesca C, Vegue Collado J, Vivas Cano MC, Pumarola Suñe T, Tórtola Fernández MT] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Aznar ML, Bocanegra Garcia C, Gil Olivas E] Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Hospital Nossa Senhora da Paz, Cubal, Angola. [Moreno MM] Hospital Nossa Senhora da Paz, Cubal, Angola. [Molina Romero I] Servei de Malalties Infeccioses, Vall d’Hebron Hospital Universitari, Barcelona, Spain. PROSICS Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain |
dc.identifier.pmid | 34641802 |
dc.identifier.wos | 000706724400001 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |