dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Silgado Gimenez, Aroa |
dc.contributor.author | Gual-Gonzalez, Lídia |
dc.contributor.author | Sánchez Montalvá, Adrián |
dc.contributor.author | Oliveira Souto, Ines Mercedes |
dc.contributor.author | Goterris Bonet, Lidia |
dc.contributor.author | Serre Delcor, Nuria |
dc.contributor.author | Esperalba Esquerra, Juliana |
dc.contributor.author | Fernandez Naval, Candela |
dc.contributor.author | Pumarola Suñé, Tomàs |
dc.contributor.author | Sulleiro Igual, Elena |
dc.contributor.author | Gómez i Prat, Jordi |
dc.contributor.author | Molina Romero, Israel |
dc.date.accessioned | 2022-05-04T10:34:10Z |
dc.date.available | 2022-05-04T10:34:10Z |
dc.date.issued | 2021-09-15 |
dc.identifier.citation | Silgado A, Gual-Gonzalez L, Sánchez-Montalvá A, Oliveira-Souto I, Goterris L, Serre-Delcor N, et al. Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease. Front Cell Infect Microbiol. 2021 Sep 15;11:736630. |
dc.identifier.issn | 2235-2988 |
dc.identifier.uri | https://hdl.handle.net/11351/7476 |
dc.description | Trypanosoma cruzi; Dried blood spot (DBS); Serological screening |
dc.description.abstract | Background: Chagas disease is a public health problem not only in Latin America, but also in other regions, including Spain, due to migration movements. Conventional serological diagnosis requires an invasive sample (plasma or serum) and a well-equipped laboratory. To circumvent those limitations, blood samples dried on filter paper (DBS) or Rapid Diagnostic Test (RDT) could be a practical alternative to reference protocol for serological screening in epidemiological studies. We evaluated the usefulness of dried blood sampling and a rapid diagnostic test (Trypanosoma Detect™) for the detection of antibodies against T. cruzi for their use in community-based screening.
Methodology/Principal Findings: A total of 162 stored paired whole-blood and serum samples from Latin American migrants and 25 negative-control blood samples were included. Diagnosis of chronic Chagas disease was performed in serum according to WHO algorithms. Blood samples were retrospectively collected as dried spots and then analyzed using two different serological techniques, enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (E-CLIA). Whole-blood samples were also used to evaluate a rapid diagnostic test based on immunochromatography. A better correlation with conventional serum was observed in dried blood elutes using E-CLIA than ELISA (97% vs. 77% sensitivity, respectively). Both assays reported 100% specificity. The median cut-off index values of E-CLIA for dried blood were significantly lower than those for serum (138.1 vs. 243.3, P<0.05). The Trypanosoma Detect™ test presented a sensitivity and specificity of 89.6% and 100%, respectively.
Conclusions: The detection of antibodies against T. cruzi in dried blood samples shows a higher sensitivity when using E-CLIA compared with ELISA. Trypanosoma Detect™ is easier to use but has a lower sensitivity. Hence, we propose a sequential strategy based on performing the rapid test first, and a negative result will be confirmed by DBS-ECLIA for use in community Chagas disease screening programs. |
dc.language.iso | eng |
dc.publisher | Frontiers Media |
dc.relation.ispartofseries | Frontiers in Cellular and Infection Microbiology;11 |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | Chagas, Malaltia de - Diagnòstic |
dc.subject | Sang - Anàlisi |
dc.subject | Serodiagnòstic |
dc.subject.mesh | Chagas Disease |
dc.subject.mesh | /diagnosis |
dc.subject.mesh | Diagnostic Tests, Routine |
dc.subject.mesh | Dried Blood Spot Testing |
dc.title | Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.3389/fcimb.2021.736630 |
dc.subject.decs | enfermedad de Chagas |
dc.subject.decs | /diagnóstico |
dc.subject.decs | pruebas diagnósticas rutinarias |
dc.subject.decs | análisis de manchas de sangre seca |
dc.relation.publishversion | https://doi.org/10.3389/fcimb.2021.736630 |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Silgado A, Goterris L, Esperalba J, Fernández-Naval C, Pumarola T, Sulleiro E] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. PROSICS Barcelona, Barcelona, Spain. [Gual-Gonzalez L] Laboratory of Vector-Borne and Zoonotic Diseases, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States. [Sánchez-Montalvá A, Oliveira-Souto I, Serre-Delcor N, Gomez-I-Prat J, Molina I] Servei de Malalties Infeccioses, Drassanes - Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. PROSICS Barcelona, Barcelona, Spain |
dc.identifier.pmid | 34604116 |
dc.identifier.wos | 000701205100001 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |