dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Funda, Jiřı́ |
dc.contributor.author | Villena Delgado, Josep A |
dc.contributor.author | Bardova, Kristina |
dc.contributor.author | Adamcova, Katerina |
dc.contributor.author | Irodenko, Illaria |
dc.contributor.author | Flachs, Pavel |
dc.date.accessioned | 2022-09-09T08:13:25Z |
dc.date.available | 2022-09-09T08:13:25Z |
dc.date.issued | 2022-04 |
dc.identifier.citation | Funda J, Villena JA, Bardova K, Adamcova K, Irodenko I, Flachs P, et al. Adipose tissue-specific ablation of PGC-1β impairs thermogenesis in brown fat. Dis Model Mech. 2022 Apr;15(4):dmm049223. |
dc.identifier.issn | 1754-8411 |
dc.identifier.uri | https://hdl.handle.net/11351/8092 |
dc.description | Adrenergic control; Lipid metabolism; Mice |
dc.description.abstract | Impaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β; officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue; BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1β knockout mice (PGC-1β-AT-KO mice) we aimed to learn whether specific PGC-1β ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30°C) mutant mice were relatively sensitive to acute cold exposure (6°C). When these mice were subjected to cold exposure for 7 days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1β-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1β-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1β in controlling BAT lipid metabolism and thermogenesis. |
dc.language.iso | eng |
dc.publisher | The Company of Biologists |
dc.relation.ispartofseries | Disease Models & Mechanisms;15(4) |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | Teixit adipós |
dc.subject | Temperatura corporal |
dc.subject | Expressió gènica |
dc.subject.mesh | Adipose Tissue, Brown |
dc.subject.mesh | Thermogenesis |
dc.subject.mesh | /genetics |
dc.title | Adipose tissue-specific ablation of PGC-1β impairs thermogenesis in brown fat |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.1242/dmm.049223 |
dc.subject.decs | grasa parda |
dc.subject.decs | termogénesis |
dc.subject.decs | /genética |
dc.relation.publishversion | https://doi.org/10.1242/dmm.049223 |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Funda J] Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic. [Villena JA] Laboratori de Metabolisme i Obesitat, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain. [Bardova K, Adamcova K, Irodenko I, Flachs P] Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic |
dc.identifier.pmid | 35466996 |
dc.identifier.wos | 000796546700007 |
dc.relation.projectid | info:eu-repo/grantAgreement/ES/PE2017-2020/RTI2018-099250-B-100 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |