dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Mariscal, Anaís |
dc.contributor.author | Zamora, Carlos |
dc.contributor.author | Diaz-Torne, Cesar |
dc.contributor.author | De Agustin De Oro, Juan Jose |
dc.contributor.author | Reina, Delia |
dc.contributor.author | Ortiz, M. Angels |
dc.date.accessioned | 2022-09-09T09:43:45Z |
dc.date.available | 2022-09-09T09:43:45Z |
dc.date.issued | 2022-05-20 |
dc.identifier.citation | Mariscal A, Zamora C, Díaz-Torné C, Ortiz MÀ, Agustín JJ, Reina D, et al. Increase of Circulating Monocyte-Platelet Conjugates in Rheumatoid Arthritis Responders to IL-6 Blockage. Int J Mol Sci. 2022 May 20;23(10):5748. |
dc.identifier.issn | 1422-0067 |
dc.identifier.uri | https://hdl.handle.net/11351/8105 |
dc.description | Monocytes; Rheumatoid arthritis; Tocilizumab |
dc.description.abstract | Platelets (PLT) bind to a significant percentage of circulating monocytes and this immunomodulatory interaction is increased in several inflammatory and autoimmune conditions. The therapeutic blockage of IL-6 with Tocilizumab (TCZ) alters PLT and the phenotype and function of monocytes in rheumatoid arthritis (RA). However, the relationship between monocyte–PLT conjugates (CD14+PLT+) and clinical and immunological variables and the regulation of this interaction by IL-6 blockage are still unknown. Here, we compared the presence of monocyte–PLT conjugates (CD14+PLT+) and membrane CD162 expression using flow cytometry, and, by ELISA, the markers of PLT activation (sCD62P and sCD40L) in healthy donors (HD) and patients with long-standing RA before TCZ (baseline). We found higher percentages and absolute counts of CD14+PLT+, and higher plasmatic levels of sCD62P and sCD40L but lower CD162 expression on monocytes from RA patients than those from HD. Additionally, the levels of CD14+PLT+ inversely correlated with inflammatory parameters. Interestingly, 95% of patients with lower percentages of CD14+PLT+ and only 63% of patients with higher percentages of CD14+PLT+ achieved a EULAR-defined response at four weeks (p = 0.036). After TCZ, the percentage of CD14+PLT+ increased in 92% of RA patients who achieved 12 w-remission (p < 0.001). Our results suggest that the binding of PLTs has a modulatory effect, accentuated by the increased binding of PLTs to monocytes in response to the therapeutic blockage of IL-6. |
dc.language.iso | eng |
dc.publisher | MDPI |
dc.relation.ispartofseries | International Journal of Molecular Sciences;23(10) |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | Artritis reumatoide - Tractament |
dc.subject | Plaquetes sanguínies |
dc.subject | Anticossos monoclonals - Ús terapèutic |
dc.subject.mesh | Arthritis, Rheumatoid |
dc.subject.mesh | /drug therapy |
dc.subject.mesh | Blood Platelets |
dc.subject.mesh | Antibodies, Monoclonal, Humanized |
dc.title | Increase of Circulating Monocyte–Platelet Conjugates in Rheumatoid Arthritis Responders to IL-6 Blockage |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.3390/ijms23105748 |
dc.subject.decs | artritis reumatoide |
dc.subject.decs | /farmacoterapia |
dc.subject.decs | plaquetas |
dc.subject.decs | anticuerpos monoclonales humanizados |
dc.relation.publishversion | https://doi.org/10.3390/ijms23105748 |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Mariscal A] Immunology Department, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain. [Zamora C, Ortiz MÀ] Laboratory of Inflammatory Diseases, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain. [Díaz-Torné C] Rheumatology Department, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. [Agustín JJ] Servei de Reumatologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Reina D] Rheumatology Department, Hospital Moisès Broggi, Sant Joan Despí, Barcelona, Spain |
dc.identifier.pmid | 35628558 |
dc.identifier.wos | 000801869500001 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |