dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Batista, Lissette |
dc.contributor.author | Robles Alonso, Virginia |
dc.contributor.author | Manichanh, Chaysavanh |
dc.contributor.author | Ruiz, Laura |
dc.contributor.author | Guagnozzi, Danila |
dc.contributor.author | Pinsach Batet, Ferran |
dc.contributor.author | Guarner Aguilar, Francisco |
dc.contributor.author | Fernández-Bañares, Fernando |
dc.date.accessioned | 2022-09-13T06:39:32Z |
dc.date.available | 2022-09-13T06:39:32Z |
dc.date.issued | 2022-06-28 |
dc.identifier.citation | Batista L, Robles V, Manichanh C, Ruiz L, Guagnozzi D, Pinsach F, et al. Colonic bacterial diversity and dysbiosis in active microscopic colitis as compared to chronic diarrhoea and healthy controls: effect of polyethylene glycol after bowel lavage for colonoscopy. BMC Gastroenterol. 2022 Jun 28;22:320. |
dc.identifier.issn | 1471-230X |
dc.identifier.uri | https://hdl.handle.net/11351/8169 |
dc.description | Faecal microbiome; Microscopic colitis; Polyethylene glycol |
dc.description.abstract | Background
Most microbiota studies in microscopic colitis patients are performed after diagnostic colonoscopy without considering the potential effect of colonic lavage. Patients may achieve clinical remission after colonoscopy and it is unknown whether lavage-induced changes play a role.
Aim
To assess the effect of polyethylene glycol (PEG) colonic lavage on clinical remission rate, microbial diversity, microbial dysbiosis index and specific microbial changes in patients with active microscopic colitis as compared to other diarrhoeal diseases and healthy controls.
Methods
Fifty-five consecutive patients presenting chronic watery diarrhoea and 12 healthy controls were included. Faecal samples were collected three days before and 30 days after PEG in patients and controls for microbiome analysis.
Results
Clinical remission was observed in 53% of microscopic colitis patients, and in 32% of non-microscopic colitis patients (p = 0.16). Considering patients with persisting diarrhoea after colonoscopy, 71% of non-microscopic colitis patients had bile acid diarrhoea. Baseline Shannon Index was lower in diarrhoea groups than in healthy controls (p = 0.0025); there were no differences between microscopic colitis, bile-acid diarrhoea and functional diarrhoea. The microbial dysbiosis index was significantly higher in microscopic colitis than in bile acid diarrhoea plus functional diarrhoea (p = 0.0095), but no bacterial species showed a significantly different relative abundance among the diarrheal groups.
Conclusions
Dysbiosis is a feature in active microscopic colitis, but loss of microbial diversity was similar in all diarrheal groups, suggesting that faecal microbial changes are not due to microscopic colitis itself but associated with stool form. A considerable number of microscopic colitis patients achieved clinical remission after colonoscopy, but we were unable to demonstrate related PEG-induced changes in faecal microbiome. |
dc.language.iso | eng |
dc.publisher | BMC |
dc.relation.ispartofseries | BMC Gastroenterology;22 |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | Diarrea - Complicacions |
dc.subject | Colonoscòpia |
dc.subject | Intestins - Microbiologia |
dc.subject.mesh | Dysbiosis |
dc.subject.mesh | Diarrhea |
dc.subject.mesh | /complications |
dc.subject.mesh | Colonoscopy |
dc.title | Colonic bacterial diversity and dysbiosis in active microscopic colitis as compared to chronic diarrhoea and healthy controls: effect of polyethylene glycol after bowel lavage for colonoscopy |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.1186/s12876-022-02392-w |
dc.subject.decs | disbacteriosis |
dc.subject.decs | diarrea |
dc.subject.decs | /complicaciones |
dc.subject.decs | colonoscopia |
dc.relation.publishversion | https://doi.org/10.1186/s12876-022-02392-w |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Batista L, Fernández-Bañares F] Department of Gastroenterology, Hospital Universitari Mutua Terrassa, Terrassa, Spain. Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain. [Robles V, Manichanh C, Guagnozzi D, Guarner F] Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain. [Ruiz L] Department of Gastroenterology, Hospital Universitari Mutua Terrassa, Terrassa, Spain. [Pinsach F] Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain |
dc.identifier.pmid | 35764931 |
dc.identifier.wos | 000818999200002 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |