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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorRamos, David
dc.contributor.authorCatita, Joana
dc.contributor.authorLópez-Luppo, Mariana
dc.contributor.authorValença, Andreia
dc.contributor.authorBonet, Aina
dc.contributor.authorCarretero, Ana
dc.contributor.authorMeseguer Navarro, Anna
dc.date.accessioned2019-02-19T09:33:47Z
dc.date.available2019-02-19T09:33:47Z
dc.date.issued2019-02
dc.identifier.citationRamos D, Catita J, López-Luppo M, Valença A, Bonet A, Carretero A, et al. Vascular Interstitial Cells in Retinal Arteriolar Annuli Are Altered During Hypertension. Invest Ophthalmol Vis Sci. 2019;60(2):473-487.
dc.identifier.issn0146-0404
dc.identifier.urihttp://hdl.handle.net/11351/3792
dc.descriptionHypertension; Interstitial cells; Arteriolar annuli
dc.description.abstractPurpose: It has been suggested that arteriolar annuli localized in retinal arterioles regulate retinal blood flow acting as sphincters. Here, the morphology and protein expression profile of arteriolar annuli have been analyzed under physiologic conditions in the retina of wild-type, β-actin-Egfp, and Nestin-gfp transgenic mice. Additionally, to study the effect of hypertension, the KAP transgenic mouse has been used. Methods: Cellular architecture has been studied using digested whole mount retinas and transmission electron microscopy. The profile of protein expression has been analyzed on paraffin sections and whole mount retinas by immunofluorescence and histochemistry. Results: The ultrastructural analysis of arteriolar annuli showed a different cell population found between endothelial and muscle cells that matched most of the morphologic criteria established to define interstitial Cajal cells. The profile of protein expression of these vascular interstitial cells (VICs) was similar to that of interstitial Cajal cells and different from the endothelial and smooth muscle cells, because they expressed β-actin, nestin, and CD44, but they did not express CD31 and α-SMA or scarcely express F-actin. Furthermore, VICs share with pericytes the expression of NG2 and platelet-derived growth factor receptor beta (PDGFR-β). The high expression of Ano1 and high activity of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase observed in VICs was diminished during hypertensive retinopathy suggesting that these cells might play a role on the motility of arteriolar annuli and that this function is altered during hypertension. Conclusions: A novel type of VICs has been described in the arteriolar annuli of mouse retina. Remarkably, these cells undergo important molecular modifications during hypertensive retinopathy and might thus be a therapeutic target against this disease.
dc.language.isoeng
dc.publisherAssociation for Research in Vision and Ophthalmology
dc.relation.ispartofseriesInvestigative Ophthalmology & Visual Science;60(2)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceScientia
dc.subjectHipertensió
dc.subjectArtèries - Citologia
dc.subjectRetina - Citologia
dc.subject.meshArteries
dc.subject.mesh/cytology
dc.subject.meshHypertension
dc.subject.meshRetina
dc.subject.mesh/cytology
dc.titleVascular Interstitial Cells in Retinal Arteriolar Annuli Are Altered During Hypertension
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1167/iovs.18-25000
dc.subject.decsarterias
dc.subject.decs/citología
dc.subject.decshipertensión
dc.subject.decsretina
dc.subject.decs/citología
dc.relation.publishversionhttps://iovs.arvojournals.org/article.aspx?articleid=2723704
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Ramos D] CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Universidade de Lisboa, Lisbon, Portugal. Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Catita J] Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona, Bellaterra, Spain. Department of Animal Health and Anatomy, School of Veterinary Medicine, Universitat Autònoma de Barcelona, Bellaterra, Spain. Department of Anatomy, Faculty of Veterinary Medicine, Universidade Lusófona de Humanidades e Tecnologias, Lisbon, Portugal. [López-Luppo M] Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona, Bellaterra, Spain. Department of Animal Health and Anatomy, School of Veterinary Medicine, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Valenca A] CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Universidade de Lisboa, Lisbon, Portugal. Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Bonet A] Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona, Bellaterra, Spain. Department of Animal Health and Anatomy, School of Veterinary Medicine, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Carretero A] CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Universidade de Lisboa, Lisbon, Portugal. Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de Barcelona, Bellaterra, Spain. Department of Animal Health and Anatomy, School of Veterinary Medicine, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Meseguer A] Grup de fisiopatologia renal, Vall d’Hebron Institut de Recerca, Barcelona, Spain. Department of Biochemistry and Molecular Biology, Unitat de Bioquímica de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Red de Investigación Renal (REDINREN), Instituto Carlos III-FEDER, Madrid, Spain.
dc.identifier.pmid30707220
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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