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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorDopico, Eva
dc.contributor.authorPimenta del Rei, Rodrigo
dc.contributor.authorEspinoza, Bertha
dc.contributor.authorUbillos, Itziar
dc.contributor.authorZanchin, Nilson Ivo Tonin
dc.contributor.authorSulleiro Igual, Elena
dc.contributor.authorMoure Garcia, Zaira
dc.date.accessioned2019-05-07T07:01:33Z
dc.date.available2019-05-07T07:01:33Z
dc.date.issued2019-03-12
dc.identifier.citationDopico E, Del-Rei RP, Espinoza B, Ubillos I, Zanchin NIT, Sulleiro E, et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infect Dis. 2019;19(1):251.
dc.identifier.issn1471-2334
dc.identifier.urihttp://hdl.handle.net/11351/4056
dc.descriptionChagas disease; Chimeric antigens; Trypanosoma cruzi
dc.description.abstractBACKGROUND: Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. METHODS: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. RESULTS: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. CONCLUSIONS: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in non-endemic settings.
dc.language.isoeng
dc.publisherBMC
dc.relation.ispartofseriesBMC Infectious Diseases;19(1)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceScientia
dc.subjectAntígens - Immunologia
dc.subjectChagas, Malaltia de - Barcelona
dc.subjectImmigrants - Malalties - Diagnòstic
dc.subject.meshAntigens, Protozoan
dc.subject.meshChagas Disease
dc.subject.meshEmigrants and Immigrants
dc.subject.mesh/statistics & numerical data
dc.subject.meshBarcelona
dc.titleImmune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1186/s12879-019-3872-z
dc.subject.decsantígenos protozoarios
dc.subject.decsenfermedad de Chagas
dc.subject.decsemigrantes e inmigrantes
dc.subject.decs/estadística & datos numéricos
dc.subject.decsBarcelona
dc.relation.publishversionhttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-019-3872-z
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Dopico E, Ubillos I] Laboratori Clínic Territorial Metropolitana Sud, Institut Català de la Salut, Barcelona, Spain. [Del-Rei RP] Faculty of Technology and Sciences of Bahia, Salvador, Bahia, Brazil. [Espinoza B] Instituto de Investigaciones Biomédicas, Departamento de Inmunología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. [Zanchin NIT] Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil. [Sulleiro E, Moure Z] Servei de Microbiologia, Hospital Universitari Vall d'Hebron, Barcelona, Spain.
dc.identifier.pmid30871504
dc.identifier.wosWOS:000461371800002
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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