Show simple item record

 
dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorRomán Canal, Berta
dc.contributor.authorMoiola, Cristian Pablo
dc.contributor.authorGatius, Sònia
dc.contributor.authorBonnin, Sarah
dc.contributor.authorRuiz-Miró, Maria
dc.contributor.authorGonzález, Esperanza
dc.contributor.authorGil Moreno, Antonio
dc.contributor.authorColas Ortega, Eva
dc.date.accessioned2019-08-07T06:27:20Z
dc.date.available2019-08-07T06:27:20Z
dc.date.issued2019-06-18
dc.identifier.citationRoman-Canal B, Moiola CP, Gatius S, Bonnin S, Ruiz-Miró M, González E, et al. EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer. Cancers (Basel). 2019;11(6):e839.
dc.identifier.issn2072-6694
dc.identifier.urihttp://hdl.handle.net/11351/4250
dc.descriptionAscitic fluid; Biomarkers; Endometrial cancer
dc.description.abstractEndometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC.
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesCancers;11(6)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectEndometri - Càncer - Diagnòstic
dc.subjectMicroARN
dc.subjectMarcadors tumorals
dc.subject.meshEndometrial Neoplasms
dc.subject.mesh/diagnosis
dc.subject.meshMicroRNAs
dc.subject.meshBiomarkers, Tumor
dc.titleEV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/cancers11060839
dc.subject.decsneoplasias endometriales
dc.subject.decs/diagnóstico
dc.subject.decsmicroARN
dc.subject.decsmarcadores tumorales
dc.relation.publishversionhttps://www.mdpi.com/2072-6694/11/6/839
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Roman-Canal B] Departament de Patologia i Genètica Molecular, Grup de Patologia Oncològica, Institut de Recerca Biomèdica de Lleida, Lleida, Spain. Universitat de Lleida, Lleida, Spain. Departament de Patologia, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Spain. Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona, Spain. Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Moiola CP] Departament de Patologia i Genètica Molecular, Grup de Patologia Oncològica, Institut de Recerca Biomèdica de Lleida, Lleida, Spain. Universitat de Lleida, Lleida, Spain. Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain. [Gatius S, Ruiz-Miró M] Departament de Patologia i Genètica Molecular, Grup de Patologia Oncològica, Institut de Recerca Biomèdica de Lleida, Lleida, Spain. Universitat de Lleida, Lleida, Spain. [Bonnin S] Centre de Regulació Genòmica (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain. [González E] Exosomes Laboratory and Metabolomics Platform. CIC bioGUNE, CIBEREHD Bizkaia Technology Park, Derio, Spain. [Gil-Moreno A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. Servei de Ginecologia Oncològica, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Colas E] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca, Barcelona, Spain
dc.identifier.pmid31216648
dc.identifier.wosWOS:000475351200103
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record