Show simple item record

 
dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorNavinés-Ferrer, Arnau
dc.contributor.authorAinsua-Enrich, Erola
dc.contributor.authorSerrano-Candelas, Eva
dc.contributor.authorSayos Ortega, Juan
dc.contributor.authorMartin, Margarita
dc.date.accessioned2019-08-09T06:48:04Z
dc.date.available2019-08-09T06:48:04Z
dc.date.issued2019-05-09
dc.identifier.citationNavinés-Ferrer A, Ainsua-Enrich E, Serrano-Candelas E, Sayós J, Martin M. Myo1f, an Unconventional Long-Tailed Myosin, Is a New Partner for the Adaptor 3BP2 Involved in Mast Cell Migration. Front Immunol. 2019;10:1058.
dc.identifier.issn1664-3224
dc.identifier.urihttp://hdl.handle.net/11351/4257
dc.descriptionKIT signaling; Adaptor molecules; Cell migration and adhesion
dc.description.abstractMast cell chemotaxis is essential for cell recruitment to target tissues, where these cells play an important role in adaptive and innate immunity. Stem cell factor (SCF) is a major chemoattractant for mast cells. SCF binds to the KIT receptor, thereby triggering tyrosine phosphorylation in the cytoplasmic domain and resulting in docking sites for SH2 domain-containing molecules, such as Lyn and Fyn, and the subsequent activation of the small GTPases Rac that are responsible for cytoskeletal reorganization and mast cell migration. In previous works we have reported the role of 3BP2, an adaptor molecule, in mast cells. 3BP2 silencing reduces FcεRI-dependent degranulation, by targeting Lyn and Syk phosphorylation, as well as SCF-dependent cell survival. This study examines its role in SCF-dependent migration and reveals that 3BP2 silencing in human mast cell line (LAD2) impairs cell migration due to SCF and IgE. In that context we found that 3BP2 silencing decreases Rac-2 and Cdc42 GTPase activity. Furthermore, we identified Myo1f, an unconventional type-I myosin, as a new partner for 3BP2. This protein, whose functions have been described as critical for neutrophil migration, remained elusive in mast cells. Myo1f is expressed in mast cells and colocalizes with cortical actin ring. Interestingly, Myo1f-3BP2 interaction is modulated by KIT signaling. Moreover, SCF dependent adhesion and migration through fibronectin is decreased after Myo1f silencing. Furthermore, Myo1f silencing leads to downregulation of β1 and β7 integrins on the mast cell membrane. Overall, Myo1f is a new 3BP2 ligand that connects the adaptor to actin cytoskeleton and both molecules are involved in SCF dependent mast cell migration.
dc.language.isoeng
dc.publisherFrontiers Media
dc.relation.ispartofseriesFrontiers in Immunology;10
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectCèl·lules - Motilitat
dc.subjectMiosina
dc.subjectProteïnes portadores
dc.subject.meshCell Movement
dc.subject.mesh/physiology
dc.subject.meshMyosin Type I
dc.subject.meshAdaptor Proteins, Signal Transducing
dc.titleMyo1f, an Unconventional Long-Tailed Myosin, Is a New Partner for the Adaptor 3BP2 Involved in Mast Cell Migration
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3389/fimmu.2019.01058
dc.subject.decsmovimiento celular
dc.subject.decs/fisiología
dc.subject.decsmiosina de tipo I
dc.subject.decsproteínas adaptadoras transductoras de señales
dc.relation.publishversionhttps://www.frontiersin.org/articles/10.3389/fimmu.2019.01058/full
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Navinés-Ferrer A, Ainsua-Enrich E, Serrano-Candelas E, Martin M] Unitat de Bioquimica, Departament de Biomedicina, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain. Laboratori de Immunoal•lèrgia Respiratòria Clínica i Experimental, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain. [Sayós J] Regulació Immunològica i Immunoteràpia, CIBBIM-Nanomedicina, Vall d’Hebron Institut de Recerca, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain.
dc.identifier.pmid31143189
dc.identifier.wosWOS:000467441300001
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record