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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorArikan Abello, Fuat
dc.contributor.authorMartínez Valverde, Tamara
dc.contributor.authorSánchez Guerrero, Ángela
dc.contributor.authorCampos Martorell, Mireia
dc.contributor.authorEsteves Coelho, Marielle
dc.contributor.authorGándara Sabatini, Dario Fabian
dc.contributor.authorTorne Torne, Ramon
dc.contributor.authorCastro González, Lidia
dc.contributor.authorSahuquillo Barris, Joan
dc.date.accessioned2021-04-27T11:46:56Z
dc.date.available2021-04-27T11:46:56Z
dc.date.issued2017-02-24
dc.identifier.citationArikan F, Martínez-Valverde T, Sánchez-Guerrero Á, Campos M, Esteves M, Gandara D, et al. Malignant infarction of the middle cerebral artery in a porcine model. A pilot study. Baron J-C, editor. PLoS One. 2017 Feb 24;12(2):e0172637.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11351/5915
dc.descriptionAnimal models; Central nervous system; Infarction
dc.description.abstractBackground and purpose Interspecies variability and poor clinical translation from rodent studies indicate that large gyrencephalic animal stroke models are urgently needed. We present a proof-of-principle study describing an alternative animal model of malignant infarction of the middle cerebral artery (MCA) in the common pig and illustrate some of its potential applications. We report on metabolic patterns, ionic profile, brain partial pressure of oxygen (PtiO2), expression of sulfonylurea receptor 1 (SUR1), and the transient receptor potential melastatin 4 (TRPM4). Methods A 5-hour ischemic infarct of the MCA territory was performed in 5 2.5-to-3-month-old female hybrid pigs (Large White x Landrace) using a frontotemporal approach. The core and penumbra areas were intraoperatively monitored to determine the metabolic and ionic profiles. To determine the infarct volume, 2,3,5-triphenyltetrazolium chloride staining and immunohistochemistry analysis was performed to determine SUR1 and TRPM4 expression. Results PtiO2 monitoring showed an abrupt reduction in values close to 0 mmHg after MCA occlusion in the core area. Hourly cerebral microdialysis showed that the infarcted tissue was characterized by reduced concentrations of glucose (0.03 mM) and pyruvate (0.003 mM) and increases in lactate levels (8.87mM), lactate-pyruvate ratio (4202), glycerol levels (588 μM), and potassium concentration (27.9 mmol/L). Immunohistochemical analysis showed increased expression of SUR1-TRPM4 channels. Conclusions The aim of the present proof-of-principle study was to document the feasibility of a large animal model of malignant MCA infarction by performing transcranial occlusion of the MCA in the common pig, as an alternative to lisencephalic animals. This model may be useful for detailed studies of cerebral ischemia mechanisms and the development of neuroprotective strategies.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPloS ONE;12(2)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectInfart cerebral
dc.subjectModels animals en la investigació
dc.subject.meshInfarction, Middle Cerebral Artery
dc.subject.meshDisease Models, Animal
dc.titleMalignant infarction of the middle cerebral artery in a porcine model. A pilot study
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1371/journal.pone.0172637
dc.subject.decsinfarto de la arteria cerebral media
dc.subject.decsmodelos de enfermedad en animales
dc.relation.publishversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0172637
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Arikan F, Torné R, Sahuquillo J] Servei de Neurocirurgia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Unitat de Recerca en Neurotraumatologia i Neurocirurgia UNINN, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Martínez-Valverde T, Sánchez-Guerrero Á, Castro L] Unitat de Recerca en Neurotraumatologia i Neurocirurgia UNINN, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Campos M, Esteves M] Unitat de Cirurgia Experimental, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain
dc.identifier.pmid28235044
dc.identifier.wos000394688200106
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/1PN/2008-2011/PI11%2F00700
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/2PN/2008-2011/FI12%2F00074
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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