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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorMartínez-Torró, Carlos
dc.contributor.authorTorres-Puig, Sergi
dc.contributor.authorMonge Azemar, Marta
dc.contributor.authorSánchez-Alba, Lucía
dc.contributor.authorGonzález-Martín, Miguel
dc.contributor.authorMarcos-Silva, Marina
dc.contributor.authorCanals Suris, Francesc
dc.date.accessioned2021-09-09T06:20:30Z
dc.date.available2021-09-09T06:20:30Z
dc.date.copyright2019
dc.date.issued2020-12-20
dc.identifier.citationMartínez-Torró C, Torres-Puig S, Monge M, Sánchez-Alba L, González-Martín M, Marcos-Silva M, et al. Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways. Emerg Microbes Infect. 2020 Dec 20;9:5-9.
dc.identifier.issn2222-1751
dc.identifier.urihttp://hdl.handle.net/11351/6284
dc.descriptionFerric uptake regulator; Mycoplasma genitalium; Emerging STI pathogen
dc.description.abstractTransition metals participate in numerous enzymatic reactions and they are essential for survival in all living organisms. For this reason, bacterial pathogens have evolved dedicated machineries to effectively compete with their hosts and scavenge metals at the site of infection. In this study, we investigated the mechanisms controlling metal acquisition in the emerging human pathogen Mycoplasma genitalium. We observed a robust transcriptional response to metal starvation, and many genes coding for predicted lipoproteins and ABC-transporters were significantly up-regulated. Transcriptional analysis of a mutant strain lacking a metalloregulator of the Fur family revealed the activation of a full operon encoding a putative metal transporter system and a gene coding for a Histidine-rich lipoprotein (Hrl). We recognized a conserved sequence with dyad symmetry within the promoter region of the Fur-regulated genes. Mutagenesis of the predicted Fur operator within the hrl promoter abrogated Fur- and metal-dependent expression of a reporter gene. Metal starvation still impelled a strong transcriptional response in the fur mutant, demonstrating the existence of Fur-independent regulatory pathways controlling metal homeostasis. Finally, analysis of metal accumulation in the wild-type strain and the fur mutant by ICP-MS revealed an important role of Fur in nickel acquisition.
dc.language.isoeng
dc.publisherTaylor and Francis
dc.relation.ispartofseriesEmerging Microbes & Infections;9
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectRegulació genètica
dc.subjectMalalties bacterianes gramnegatives
dc.subject.meshMycoplasma genitalium
dc.subject.meshGene Expression Regulation, Bacterial
dc.titleTranscriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1080/22221751.2019.1700762
dc.subject.decsMycoplasma genitalium
dc.subject.decsregulación de la expresión génica bacteriana
dc.relation.publishversionhttps://doi.org/10.1080/22221751.2019.1700762
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Martínez-Torró C, Torres-Puig S, Sánchez-Alba L, González-Martín M, Marcos-Silva M] Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Monge M, Canals F] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain
dc.identifier.pmid31859607
dc.identifier.wos000510381200002
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2013-2016/BIO2017-84166-R
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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