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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorSuescún-Carrero, Sandra Helena
dc.contributor.authorSalamanca-Cardozo, Lyda Pilar
dc.contributor.authorPinazo, María Jesus
dc.contributor.authorArmadans Gil, Lluis
dc.date.accessioned2022-01-13T17:59:54Z
dc.date.available2022-01-13T17:59:54Z
dc.date.issued2021-06-02
dc.identifier.citationSuescún-Carrero SH, Salamanca-Cardozo LP, Pinazo MJ, Armadans-Gil L. Sensitivity and Specificity of two rapid tests for the diagnosis of infection by Trypanosoma cruzi in a Colombian population. PLoS Negl Trop Dis. 2021 Jun 2;15(6):e0009483.
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/11351/6798
dc.descriptionTrypanosoma cruzi; Parasitic diseases; Serodiagnosis
dc.description.abstractObjective To evaluate diagnostic precision of two rapid diagnostic tests (RDT’s) on patients with chronic Chagas disease. Methodology Prospective study with the following inclusion criteria: subjects older than 3 years, signed informed consent. Exclusion criterion: subjects could not have previously received treatment for infection with T. cruzi. The study population were participants in a screening process undertaken in rural and urban zones of the department Boyacá, Colombia. Two RDT’s were performed to all participants: the Chagas Detect Plus InBios (CDP) and the Chagas Stat-Pak (CSP) and as a reference standard the ELISA Chagas III GrupoBios and the Chagas ELISA IgG+IgM I Vircell tests were used. In the case of discordant results between the two ELISA tests, an indirect immunofluorescence was done. Results Three hundred-five (305) subjects were included in the study (38 patients with leishmaniasis), of which 215 tested negative for T cruzi and 90 tested positive according to the reference standard. The sensitivity of the RDT’s were 100% (CI 95% 95.9–100), and the specificity of the CDP was 99.1% (CI 95% 96.6–99.8) and for CSP was 100% (CI 95% 98.3–100). The agreement of CDP was 99.5% and for CSP was 100% with Kappa values of (k = 99.1; CI 95% 92.6–99.8%) and (k = 100; CI 95% 94.3–100), respectively. RDT’s did not present cross-reactions with samples from patients who were positive for leishmaniasis. Conclusions The findings demonstrate excellent results from the RDT’s in terms of validity, safety, and reproducibility. The results obtained provide evidence for the recommendation for using these tests in a Colombian epidemiological context principally in endemic areas in which laboratory installations necessary to perform conventional tests are not available, or they are scarce and to help in diagnosing chronic Chagas disease in order to provide access to treatment as soon as possible.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS Neglected Tropical Diseases;15(6)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectMalalties parasitàries - Epidemiologia
dc.subjectMalalties parasitàries - Diagnòstic
dc.subject.meshTrypanosoma cruzi
dc.subject.mesh/isolation & purification
dc.subject.meshSensitivity and Specificity
dc.subject.meshDiagnostic Tests, Routine
dc.titleSensitivity and Specificity of two rapid tests for the diagnosis of infection by Trypanosoma cruzi in a Colombian population
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1371/journal.pntd.0009483
dc.subject.decsTrypanosoma cruzi
dc.subject.decs/aislamiento & purificación
dc.subject.decssensibilidad y especificidad
dc.subject.decspruebas diagnósticas rutinarias
dc.relation.publishversionhttps://doi.org/10.1371/journal.pntd.0009483
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Suescún-Carrero SH] Grupo de Investigación del Laboratorio de Salud Pública de Boyacá, Secretaria de Salud de Boyacá, Tunja, Colombia, Doctorado en Metodología de la Investigación Biomédica y Salud Pública, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Salamanca-Cardozo LP] Grupo de Investigación del Laboratorio de Salud Pública de Boyacá, Secretaria de Salud de Boyacá, Tunja, Colombia. [Pinazo MJ] Barcelona Institute for Global Health (ISGlobal), Hospital Clinic—University of Barcelona, Barcelona, Spain. [Armadans-Gil L] Servei de Medicina Preventiva i Epidemiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain
dc.identifier.pmid34077424
dc.identifier.wos000664520500004
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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