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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorSamarkanova, Dinara
dc.contributor.authorCox, Steven
dc.contributor.authorHernandez, Diana
dc.contributor.authorRodriguez, Luciano
dc.contributor.authorPérez, Maria Luisa
dc.contributor.authorMadrigal, Alejandro
dc.contributor.authorQuerol Giner, Sergi
dc.date.accessioned2022-03-25T07:23:15Z
dc.date.available2022-03-25T07:23:15Z
dc.date.issued2021-07-02
dc.identifier.citationSamarkanova D, Cox S, Hernandez D, Rodriguez L, Pérez ML, Madrigal A, et al. Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties. Sci Rep. 2021 Jul 2;11:13754.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11351/7265
dc.descriptionImmunology; Medical research
dc.description.abstractDiseases and injuries that compromise the ocular surface cause considerable patient distress and have long term consequences for their quality of life. Treatment modalities that can address the delicate balance of tissue regeneration, inflammation and maintenance of corneal transparency are therefore needed. We have recently formulated two novel eye drops from placental tissues: cord blood platelet lysate (CBED) and amniotic membrane extract eye drops (AMED), which can be used to treat severe ocular disorders. Here we characterise these two preparations by measuring: (a) growth factors (GF) and cytokines composition, (b) promotion of human corneal epithelial cell (HCEC) growth and (c) effects on immune cells in a lymphocyte culture assay. Finally, their bioavailability was assayed in an ex vivo porcine corneal model. We show that both preparations contain GF and cytokines that were able to promote the in vitro growth of HCEC and support repair in an in vitro scratch test. When assessed in a lymphocyte culture, both favoured immune suppression reducing the cellular expression of NKG2D and CD107a as well as the production of interferon gamma (IFN-γ) in natural killer, NKT and T cells. Regarding bioavailability, CBED active molecules were found mainly in the pre-corneal fraction with some penetration into the corneal fraction, in an ex vivo model. In summary, both placental-derived allogeneic preparations, CBED and AMED, display regenerative and immunomodulatory capabilities. These results will help define mechanisms of action and the best indications and doses of each product for use in a particular patient and support the development of off-the-shelf therapies for ocular surface pathologies in which wound healing defects and inflammatory events are contributing factors.
dc.language.isoeng
dc.publisherNature Research
dc.relation.ispartofseriesScientific Reports;11
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectCòrnia - Malalties - Tractament
dc.subjectFarmacologia ocular
dc.subjectSang fetal - Ús terapèutic - Eficàcia
dc.subject.meshCorneal Diseases
dc.subject.mesh/drug therapy
dc.subject.meshOphthalmic Solutions
dc.subject.meshFetal Blood
dc.titleCord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1038/s41598-021-93150-7
dc.subject.decsenfermedades de la córnea
dc.subject.decs/farmacoterapia
dc.subject.decssoluciones oftálmicas
dc.subject.decssangre fetal
dc.relation.publishversionhttps://doi.org/10.1038/s41598-021-93150-7
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Samarkanova D] Banc de Sang i Teixits, 08005 Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Cox S, Hernandez D] Anthony Nolan Research Institute, Royal Free Hospital, London, UK. Barcelona Tissue Bank, Banc de Sang i Teixits, Barcelona, Spain. [Rodriguez L] Banc de Sang i Teixits, 08005 Barcelona, Spain. [Pérez ML] Banc de Sang i Teixits, 08005 Barcelona, Spain. Barcelona Tissue Bank, Banc de Sang i Teixits, Barcelona, Spain. Regenerative Medicine and Tissue Bioengineering Research Group (SGR 1113), Biomedical Research Institute (IIB-Sant Pau), Barcelona, Spain. [Madrigal A] Banc de Sang i Teixits, 08005 Barcelona, Spain. Anthony Nolan Research Institute, Royal Free Hospital, London, UK. UCL Cancer Institute, Royal Free Campus, London, UK. [Querol S] Banc de Sang i Teixits, 08005 Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain
dc.identifier.pmid34215804
dc.identifier.wos000672163000030
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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