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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorAubets, Eva
dc.contributor.authorChillon Rodriguez, Miguel
dc.contributor.authorCiudad, Carlos J.
dc.contributor.authorNoé, Véronique
dc.date.accessioned2022-04-25T12:59:39Z
dc.date.available2022-04-25T12:59:39Z
dc.date.issued2021-09
dc.identifier.citationAubets E, Chillon M, Ciudad CJ, Noé V. PolyPurine Reverse Hoogsteen Hairpins Work as RNA Species for Gene Silencing. Int J Mol Sci. 2021 Sep;22(18):10025.
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/11351/7395
dc.descriptionAdenovirus; Cancer therapy; Viral vectors
dc.description.abstractPolyPurine Reverse Hoogsteen Hairpins (PPRHs) are gene-silencing DNA-oligonucleotides developed in our laboratory that are formed by two antiparallel polypurine mirror repeat domains bound intramolecularly by Hoogsteen bonds. The aim of this work was to explore the feasibility of using viral vectors to deliver PPRHs as a gene therapy tool. After treatment with synthetic RNA, plasmid transfection, or viral infection targeting the survivin gene, viability was determined by the MTT assay, mRNA was determined by RT-qPCR, and protein levels were determined by Western blot. We showed that the RNA-PPRH induced a decrease in cell viability in a dose-dependent manner and an increase in apoptosis in PC-3 and HeLa cells. Both synthetic RNA-PPRH and RNA-PPRH intracellularly generated upon the transfection of a plasmid vector were able to reduce survivin mRNA and protein levels in PC-3 cells. An adenovirus type-5 vector encoding the PPRH against survivin was also able to decrease survivin mRNA and protein levels, leading to a reduction in HeLa cell viability. In this work, we demonstrated that PPRHs can also work as RNA species, either chemically synthesized, transcribed from a plasmid construct, or transcribed from viral vectors. Therefore, all these results are the proof of principle that viral vectors could be considered as a delivery system for PPRHs.
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesInternational Journal of Molecular Sciences;22(18)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectProteïnes - Inhibidors
dc.subjectEpigenètica
dc.subjectOligonucleòtids
dc.subject.meshOligonucleotides
dc.subject.meshGene Silencing
dc.subject.meshSurvivin
dc.titlePolyPurine Reverse Hoogsteen Hairpins Work as RNA Species for Gene Silencing
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/ijms221810025
dc.subject.decsoligonucleótidos
dc.subject.decssilenciamiento génico
dc.subject.decssurvivina
dc.relation.publishversionhttps://doi.org/10.3390/ijms221810025
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Aubets E, Ciudad CJ, Noé V] Department of Biochemistry and Physiology, School of Pharmacy and Food Sciences, Nanoscience and Nanotechnology Institute, IN2UB, University of Barcelona, 08028 Barcelona, Spain. [Chillon M] ICREA, Institut de Neurociències, Universitat Autònoma de Barcelona, Bellaterra, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain
dc.identifier.pmid34576188
dc.identifier.wos000700750700001
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2017-2020/RTI2018-093901-B-I00
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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