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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorEto, Yoshiki
dc.contributor.authorSaubí Roca, Narcís
dc.contributor.authorFerrer, Pau
dc.contributor.authorJoseph Munné, Joan
dc.date.accessioned2022-05-24T06:42:17Z
dc.date.available2022-05-24T06:42:17Z
dc.date.issued2021-11-20
dc.identifier.citationEto Y, Saubi N, Ferrer P, Joseph-Munné J. Expression of Chimeric HPV-HIV Protein L1P18 in Pichia pastoris; Purification and Characterization of the Virus-like Particles. Pharmaceutics. 2021 Nov 20;13(11):1967.
dc.identifier.issn1999-4923
dc.identifier.urihttp://hdl.handle.net/11351/7570
dc.descriptionHPV; Pichia pastoris; Purification
dc.description.abstractCurrently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38.0 million people were living with HIV in 2019, only 68% of HIV-infected individuals were accessing antiretroviral therapy as of the end of June 2020 and there is no HIV vaccine yet. Therefore, safe, effective, and affordable vaccines against those two viruses are immediately needed. Both HPV and HIV are sexually transmitted infections and one of the main access routes is the mucosal genital tract. Thus, the development of a combined vaccine that would protect against HPV and HIV infections is a logical effort in the fight against these two major global pathogens. In this study, a recombinant Pichia pastoris producing chimeric HPV-HIV L1P18 protein intracellularly was constructed. After cell disruption, the supernatant was collected, and the VLPs were purified by a combination of ammonium sulfate precipitation, size exclusion chromatography, ultracentrifugation, and ultrafiltration. At the end of purification process, the chimeric VLPs were recovered with 96% purity and 9.23% overall yield, and the morphology of VLPs were confirmed by transmission electron microscopy. This work contributes towards the development of an alternative platform for production of a bivalent vaccine against HPV and HIV in P. pastoris.
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesPharmaceutics;13(11)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectVacunes
dc.subjectMalalties de transmissió sexual - Tractament
dc.subjectPapil·lomavirus
dc.subject.meshVaccines
dc.subject.meshSexually Transmitted Diseases, Viral
dc.subject.meshPapillomavirus Infections
dc.titleExpression of Chimeric HPV-HIV Protein L1P18 in Pichia pastoris; Purification and Characterization of the Virus-like Particles
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/pharmaceutics13111967
dc.subject.decsvacunas
dc.subject.decsenfermedades virales de transmisión sexual
dc.subject.decsinfecciones por Papillomavirus
dc.relation.publishversionhttps://doi.org/10.3390/pharmaceutics13111967
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Eto Y] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament d’Enginyeria, Química, Biològica i Ambiental, Escola d’Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. AIDS Research Unit, Infectious Diseases Department, Hospital Clínic/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. [Saubi N] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. AIDS Research Unit, Infectious Diseases Department, Hospital Clínic/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. [Ferrer P] Departament d’Enginyeria, Química, Biològica i Ambiental, Escola d’Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Joseph-Munné J] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain
dc.identifier.pmid34834382
dc.identifier.wos000727904600001
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/681137
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2017-2020/PI20%2F00217
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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