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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorCuadras Solé, Mercè
dc.contributor.authorPlanas Morin, Jacques
dc.contributor.authorCelma Domènech, Ana
dc.contributor.authorRegis Plácido, Lucas
dc.contributor.authorTorres Ramirez, Inés de
dc.contributor.authorSemidey Raven, Maria Eugenia
dc.contributor.authorTrilla Herrera, Enric
dc.contributor.authorMorote Robles, Joan
dc.date.accessioned2022-08-18T08:23:51Z
dc.date.available2022-08-18T08:23:51Z
dc.date.issued2022-04-02
dc.identifier.citationCuadras M, Planas J, Celma A, Regis L, de Torres IM, Semidey ME, et al. Application of One-Step Nucleic Acid Amplification (OSNA) in different cancer entities and usefulness in prostate cancer: a systematic review. BMC Cancer. 2022 Apr 2;22:357.
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/11351/8012
dc.descriptionCytokeratin 19; Prostate cancer; Lymph node metastases
dc.description.abstractBackground Lymph node (LN) status is a key prognostic factor in the decision-making process of different cancer entities, including prostate cancer (PCa). Sectioning and haematoxylin and eosin (H&E) staining technique remain the gold standard for the evaluation of LN metastases despite some limitations, especially low sensitivity in detecting an accurate tumour burden within the LN, as well as a subjective and time-consuming result. One-step nucleic acid amplification (OSNA) quantifies mRNA copies of cytokeratin 19 (CK19) in a fast, objective, automated, and reproducible way, raising a general interest to explore its utility for lymphatic metastasis identification in different malignancies. Methods To present the latest evidence related to the detection of LN metastases in several tumours by using OSNA compared with the conventional H&E method, a systematic review of articles published since March 2021 was conducted using PubMed, Cochrane Library, and Web of Science databases. References from primary papers and review articles were checked to obtain further potential studies. Our procedure for evaluating records identified during the literature search followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses criteria. With the aim to design and justify future clinical routine use of OSNA in PCa, novel PCa evidence has been included in this review for the first time. Results Twenty five studies were included. LN from six different groups of tumours: breast, gastrointestinal, gynecological, lung, head and neck and prostate cancers has been assessed. OSNA was compared with post-operative formalin-fixed paraffin-embedded tissue sections with H&E staining as the reference standard. Contingency tables were created, and concordance rate, sensitivity, specificity and predictive values were reported. Seventeen studies analysed the discordant cases using different techniques. Conclusion OSNA method has a high diagnostic accuracy for the detection of LN metastases in several CK19 expressing tumours. Available evidence might encourage future investigations about its usage in PCa patients to improve LN staging and prognosis.
dc.language.isoeng
dc.publisherBMC
dc.relation.ispartofseriesBMC Cancer;22
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectPròstata - Càncer - Prognosi
dc.subjectGenètica - Tècnica
dc.subjectMetàstasi limfàtica
dc.subject.meshProstatic Neoplasms
dc.subject.mesh/diagnosis
dc.subject.meshNucleic Acid Amplification Techniques
dc.titleApplication of One-Step Nucleic Acid Amplification (OSNA) in different cancer entities and usefulness in prostate cancer: a systematic review
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1186/s12885-022-09355-0
dc.subject.decsneoplasias de la próstata
dc.subject.decs/diagnóstico
dc.subject.decstécnicas de amplificación de ácidos nucleicos
dc.relation.publishversionhttps://doi.org/10.1186/s12885-022-09355-0
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Cuadras M, Planas J, Celma A, Regis L, Trilla E] Servei d’Urologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [de Torres IM, Semidey ME] Servei de Patologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Morote J] Servei d’Urologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Cirurgia, Universitat Autònoma de Barcelona, Bellaterra, Spain
dc.identifier.pmid35366849
dc.identifier.wos000777432600002
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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