dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
dc.contributor.author | Tarrés Freixas, Ferran |
dc.contributor.author | Trinité, Benjamin |
dc.contributor.author | Pons-Grifols, Anna |
dc.contributor.author | Romero-Durana, Miguel |
dc.contributor.author | Riveira Muñoz, Eva |
dc.contributor.author | Ávila-Nieto, Carlos |
dc.contributor.author | Andrés Verges, Cristina |
dc.contributor.author | Antón Pagarolas, Andres |
dc.contributor.author | Pumarola Suñé, Tomàs |
dc.date.accessioned | 2022-09-07T12:04:11Z |
dc.date.available | 2022-09-07T12:04:11Z |
dc.date.issued | 2022-05 |
dc.identifier.citation | Tarrés-Freixas F, Trinité B, Pons-Grífols A, Romero-Durana M, Riveira-Muñoz E, Ávila-Nieto C, et al. Heterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice. Front Microbiol. 2022 May;13:840757. |
dc.identifier.issn | 1664-302X |
dc.identifier.uri | https://hdl.handle.net/11351/8054 |
dc.description | SARS-CoV-2; Viral load; Wildtype mice |
dc.description.abstract | The emerging SARS-CoV-2 variants of concern (VOCs) may display enhanced transmissibility, more severity and/or immune evasion; however, the pathogenesis of these new VOCs in experimental SARS-CoV-2 models or the potential infection of other animal species is not completely understood. Here we infected K18-hACE2 transgenic mice with B.1, B.1.351/Beta, B.1.617.2/Delta and BA.1.1/Omicron isolates and demonstrated heterogeneous infectivity and pathogenesis. B.1.351/Beta variant was the most pathogenic, while BA.1.1/Omicron led to lower viral RNA in the absence of major visible clinical signs. In parallel, we infected wildtype (WT) mice and confirmed that, contrary to B.1 and B.1.617.2/Delta, B.1.351/Beta and BA.1.1/Omicron can infect them. Infection in WT mice coursed without major clinical signs and viral RNA was transient and undetectable in the lungs by day 7 post-infection. In silico modeling supported these findings by predicting B.1.351/Beta receptor binding domain (RBD) mutations result in an increased affinity for both human and murine ACE2 receptors, while BA.1/Omicron RBD mutations only show increased affinity for murine ACE2. |
dc.language.iso | eng |
dc.publisher | Frontiers Media |
dc.relation.ispartofseries | Frontiers in Microbiology;13 |
dc.rights | Attribution 4.0 International |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.source | Scientia |
dc.subject | COVID-19 (Malaltia) |
dc.subject | Ratolins transgènics |
dc.subject | Simulació (Medicina) |
dc.subject.mesh | Mice, Transgenic |
dc.subject.mesh | Coronavirus Infections |
dc.subject.mesh | Computer Simulation |
dc.title | Heterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | 10.3389/fmicb.2022.840757 |
dc.subject.decs | ratones transgénicos |
dc.subject.decs | infecciones por Coronavirus |
dc.subject.decs | simulación por ordenador |
dc.relation.publishversion | https://doi.org/10.3389/fmicb.2022.840757 |
dc.type.version | info:eu-repo/semantics/publishedVersion |
dc.audience | Professionals |
dc.contributor.organismes | Institut Català de la Salut |
dc.contributor.authoraffiliation | [Tarrés-Freixas F, Trinité B, Pons-Grífols A, Riveira-Muñoz E, Ávila-Nieto C] IrsiCaixa AIDS Research Institute, Can Ruti Campus, UAB, Badalona, Spain. [Romero-Durana M] Barcelona Supercomputing Center, Barcelona, Spain. [Andrés C, Antón A, Pumarola T] Unitat de Virus Respiratoris, Servei de Microbiologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain |
dc.identifier.pmid | 35602059 |
dc.rights.accessrights | info:eu-repo/semantics/openAccess |