Show simple item record

 
dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorVandebriel, Rob
dc.contributor.authorRemy, Sylvie
dc.contributor.authorVermeulen, Jolanda P.
dc.contributor.authorHurkmans, Evelien
dc.contributor.authorKevenaar, Kirsten
dc.contributor.authorBastús, Neus G
dc.contributor.authorFranco Puntes, Victor
dc.date.accessioned2022-09-09T07:35:02Z
dc.date.available2022-09-09T07:35:02Z
dc.date.issued2022-05-20
dc.identifier.citationVandebriel RJ, Remy S, Vermeulen JP, Hurkmans EGE, Kevenaar K, Bastús NG, et al. Pathways Related to NLRP3 Inflammasome Activation Induced by Gold Nanorods. Int J Mol Sci. 2022 May 20;23(10):5763.
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/11351/8088
dc.descriptionGold; Inflammation; Nanorod
dc.description.abstractThe widespread and increasing use of engineered nanomaterials (ENM) increases the risk of human exposure, generating concern that ENM may provoke adverse health effects. In this respect, their physicochemical characteristics are critical. The immune system may respond to ENM through inflammatory reactions. The NLRP3 inflammasome responds to a wide range of ENM, and its activation is associated with various inflammatory diseases. Recently, anisotropic ENM have become of increasing interest, but knowledge of their effects on the immune system is still limited. The objective of the study was to compare the effects of gold ENM of different shapes on NLRP3 inflammasome activation and related signalling pathways. Differentiated THP-1 cells (wildtype, ASC- or NLRP3-deficient), were exposed to PEGylated gold nanorods, nanostars, and nanospheres, and, thus, also different surface chemistries, to assess NLRP3 inflammasome activation. Next, the exposed cells were subjected to gene expression analysis. Nanorods, but not nanostars or nanospheres, showed NLRP3 inflammasome activation. ASC- or NLRP3-deficient cells did not show this effect. Gene Set Enrichment Analysis revealed that gold nanorod-induced NLRP3 inflammasome activation was accompanied by downregulated sterol/cholesterol biosynthesis, oxidative phosphorylation, and purinergic receptor signalling. At the level of individual genes, downregulation of Paraoxonase-2, a protein that controls oxidative stress, was most notable. In conclusion, the shape and surface chemistry of gold nanoparticles determine NLRP3 inflammasome activation. Future studies should include particle uptake and intracellular localization.
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesInternational Journal of Molecular Sciences;23(10)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectNanopartícules
dc.subjectNanotubs
dc.subjectOr
dc.subject.meshNanotubes
dc.subject.meshInflammasomes
dc.subject.meshMetal Nanoparticles
dc.titlePathways Related to NLRP3 Inflammasome Activation Induced by Gold Nanorods
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/ijms23105763
dc.subject.decsnanotubos
dc.subject.decsinflamasomas
dc.subject.decsnanopartículas metálicas
dc.relation.publishversionhttps://doi.org/10.3390/ijms23105763
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Vandebriel RJ, Vermeulen JP, Hurkmans EGE, Kevenaar K] Centre for Health Protection, National Institute for Public Health & the Environment, Bilthoven, The Netherlands. [Remy S] Health Unit, VITO NV, Mol, Belgium. [Bastús NG] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Universitat Autònoma de Barcelona, Bellaterra, Spain. [Puntes VF] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Universitat Autònoma de Barcelona, Bellaterra, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain
dc.identifier.pmid35628574
dc.identifier.wos000803406800001
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record