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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorFunda, Jiřı́
dc.contributor.authorVillena Delgado, Josep A
dc.contributor.authorBardova, Kristina
dc.contributor.authorAdamcova, Katerina
dc.contributor.authorIrodenko, Illaria
dc.contributor.authorFlachs, Pavel
dc.date.accessioned2022-09-09T08:13:25Z
dc.date.available2022-09-09T08:13:25Z
dc.date.issued2022-04
dc.identifier.citationFunda J, Villena JA, Bardova K, Adamcova K, Irodenko I, Flachs P, et al. Adipose tissue-specific ablation of PGC-1β impairs thermogenesis in brown fat. Dis Model Mech. 2022 Apr;15(4):dmm049223.
dc.identifier.issn1754-8411
dc.identifier.urihttp://hdl.handle.net/11351/8092
dc.descriptionAdrenergic control; Lipid metabolism; Mice
dc.description.abstractImpaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β; officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue; BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1β knockout mice (PGC-1β-AT-KO mice) we aimed to learn whether specific PGC-1β ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30°C) mutant mice were relatively sensitive to acute cold exposure (6°C). When these mice were subjected to cold exposure for 7 days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1β-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1β-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1β in controlling BAT lipid metabolism and thermogenesis.
dc.language.isoeng
dc.publisherThe Company of Biologists
dc.relation.ispartofseriesDisease Models & Mechanisms;15(4)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectTeixit adipós
dc.subjectTemperatura corporal
dc.subjectExpressió gènica
dc.subject.meshAdipose Tissue, Brown
dc.subject.meshThermogenesis
dc.subject.mesh/genetics
dc.titleAdipose tissue-specific ablation of PGC-1β impairs thermogenesis in brown fat
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1242/dmm.049223
dc.subject.decsgrasa parda
dc.subject.decstermogénesis
dc.subject.decs/genética
dc.relation.publishversionhttps://doi.org/10.1242/dmm.049223
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Funda J] Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. Department of Physiology, Faculty of Science, Charles University in Prague, Prague, Czech Republic. [Villena JA] Laboratori de Metabolisme i Obesitat, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain. [Bardova K, Adamcova K, Irodenko I, Flachs P] Laboratory of Adipose Tissue Biology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
dc.identifier.pmid35466996
dc.identifier.wos000796546700007
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2017-2020/RTI2018-099250-B-100
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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