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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorMariscal, Anaís
dc.contributor.authorZamora, Carlos
dc.contributor.authorDiaz-Torne, Cesar
dc.contributor.authorOrtiz, M. Angels
dc.contributor.authorDe Agustin, Juan Jose
dc.contributor.authorReina, Delia
dc.date.accessioned2022-09-09T09:43:45Z
dc.date.available2022-09-09T09:43:45Z
dc.date.issued2022-05-20
dc.identifier.citationMariscal A, Zamora C, Díaz-Torné C, Ortiz MÀ, Agustín JJ, Reina D, et al. Increase of Circulating Monocyte-Platelet Conjugates in Rheumatoid Arthritis Responders to IL-6 Blockage. Int J Mol Sci. 2022 May 20;23(10):5748.
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/11351/8105
dc.descriptionMonocytes; Rheumatoid arthritis; Tocilizumab
dc.description.abstractPlatelets (PLT) bind to a significant percentage of circulating monocytes and this immunomodulatory interaction is increased in several inflammatory and autoimmune conditions. The therapeutic blockage of IL-6 with Tocilizumab (TCZ) alters PLT and the phenotype and function of monocytes in rheumatoid arthritis (RA). However, the relationship between monocyte–PLT conjugates (CD14+PLT+) and clinical and immunological variables and the regulation of this interaction by IL-6 blockage are still unknown. Here, we compared the presence of monocyte–PLT conjugates (CD14+PLT+) and membrane CD162 expression using flow cytometry, and, by ELISA, the markers of PLT activation (sCD62P and sCD40L) in healthy donors (HD) and patients with long-standing RA before TCZ (baseline). We found higher percentages and absolute counts of CD14+PLT+, and higher plasmatic levels of sCD62P and sCD40L but lower CD162 expression on monocytes from RA patients than those from HD. Additionally, the levels of CD14+PLT+ inversely correlated with inflammatory parameters. Interestingly, 95% of patients with lower percentages of CD14+PLT+ and only 63% of patients with higher percentages of CD14+PLT+ achieved a EULAR-defined response at four weeks (p = 0.036). After TCZ, the percentage of CD14+PLT+ increased in 92% of RA patients who achieved 12 w-remission (p < 0.001). Our results suggest that the binding of PLTs has a modulatory effect, accentuated by the increased binding of PLTs to monocytes in response to the therapeutic blockage of IL-6.
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesInternational Journal of Molecular Sciences;23(10)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectArtritis reumatoide - Tractament
dc.subjectPlaquetes sanguínies
dc.subjectAnticossos monoclonals - Ús terapèutic
dc.subject.meshArthritis, Rheumatoid
dc.subject.mesh/drug therapy
dc.subject.meshBlood Platelets
dc.subject.meshAntibodies, Monoclonal, Humanized
dc.titleIncrease of Circulating Monocyte–Platelet Conjugates in Rheumatoid Arthritis Responders to IL-6 Blockage
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/ijms23105748
dc.subject.decsartritis reumatoide
dc.subject.decs/farmacoterapia
dc.subject.decsplaquetas
dc.subject.decsanticuerpos monoclonales humanizados
dc.relation.publishversionhttps://doi.org/10.3390/ijms23105748
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Mariscal A] Immunology Department, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain. [Zamora C, Ortiz MÀ] Laboratory of Inflammatory Diseases, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona, Spain. [Díaz-Torné C] Rheumatology Department, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain. [Agustín JJ] Servei de Reumatologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Reina D] Rheumatology Department, Hospital Moisès Broggi, Sant Joan Despí, Barcelona, Spain
dc.identifier.pmid35628558
dc.identifier.wos000801869500001
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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