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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorClanxet, Jordi
dc.contributor.authorTeles, Mariana
dc.contributor.authorHernandez Losa, Javier
dc.contributor.authorRuiz‑Echarri Rueda, Manuel
dc.contributor.authorBenitez‑Fusté, Luis
dc.contributor.authorPastor, Josep
dc.date.accessioned2022-09-12T08:39:56Z
dc.date.available2022-09-12T08:39:56Z
dc.date.issued2022-05-30
dc.identifier.citationClanxet J, Teles M, Hernández-Losa J, Ruiz-Echarri Rueda M, Benitez-Fusté L, Pastor J. Gene expression profiles of beta-adrenergic receptors in canine vascular tumors: a preliminary study. BMC Vet Res. 2022 May 30;18:206.
dc.identifier.issn1746-6148
dc.identifier.urihttp://hdl.handle.net/11351/8143
dc.descriptionBeta receptors; Dog; Hemangioma
dc.description.abstractBeta adrenergic receptors (β-AR) play a key role in regulating several hallmark pathways of both benign and malignant human and canine tumors. There is scarce information on the expression of β-AR in canine vascular tumors. Therefore, the purpose of the present research work was to study the mRNA expression levels of the three subtypes of the β-AR genes (ADRB1, ADRB2, ADRB3) in hemangiosarcoma (HSA) and hemangioma (HA), as well as in vascular hamartomas (VH) from dogs. Fifty samples (n = 50) were obtained from 38 dogs. Twenty-three animals had HSA, eight animals HA and seven animals VH. HSA were auricular (n = 8), splenic (n = 5), cutaneous (n = 6), auricular and splenic (n = 2), cutaneous-muscular (n = 1) and disseminated (n = 1). There were seven cases of HSA that were divided into primary tumor and secondary (metastatic) tumor. Skin and muscle samples with a normal histological study were used as control group. ADRB gene expression was determinate in all samples by real-time quantitative PCR. Results showed that ADRB1, ADRB2 and ADRB3 were overexpressed in HSA when compared to the control group. ADRB2 was overexpressed in HA when compared to the control group. HSA express higher values of ADBR1 (p = 0.0178) compared to VH. There was a high inter-individual variability in the expression of the three subtypes of ADBR. No statistically significant difference in the expression of ADBR genes were observed between HSA primary when compared to metastatic or in different anatomical locations. In conclusion, canine HSA overexpress the three β-AR subtypes and canine HA β2-AR. High variability was observed in β-AR mRNA levels amongst HSA cases.
dc.language.isoeng
dc.publisherBMC
dc.relation.ispartofseriesBMC Veterinary Research;18
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectExpressió gènica
dc.subjectOncologia veterinària
dc.subjectGenètica veterinària
dc.subject.meshHemangiosarcoma
dc.subject.mesh/veterinary
dc.subject.meshVascular Neoplasms
dc.subject.meshGene Expression Profiling
dc.titleGene expression profiles of beta-adrenergic receptors in canine vascular tumors: a preliminary study
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1186/s12917-022-03317-1
dc.subject.decshemangiosarcoma
dc.subject.decs/veterinaria
dc.subject.decsneoplasias vasculares
dc.subject.decsperfiles de expresión génica
dc.relation.publishversionhttps://doi.org/10.1186/s12917-022-03317-1
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Clanxet J, Pastor J] Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Teles M] Departament de Biologia Cel•lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Bellaterra, Spain. Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Hernández-Losa J] Laboratori de Biologia Molecular, Servei de Patologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Ruiz-Echarri Rueda M] Bristol Royal Infrmary, Bristol, UK. [Benitez-Fusté L] Hospital Veterinari del Mar, Barcelona, Spain
dc.identifier.pmid35637463
dc.identifier.wos000805587200002
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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