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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorPardo Camacho, Cristina
dc.contributor.authorGanda Mall, John-Peter
dc.contributor.authorMartínez, Cristina
dc.contributor.authorPigrau Pastor, Marc
dc.contributor.authorExposito Fernandez, Elba
dc.contributor.authorAlbert Bayo, Merce
dc.contributor.authorMelón Ardanaz, Elisa
dc.contributor.authorNieto Ruiz, Adoración
dc.contributor.authorRodiño Janeiro, Bruno Kotska
dc.contributor.authorFortea Guillamon, Marina
dc.contributor.authorGuagnozzi, Danila
dc.contributor.authorRodríguez Urrutia, Amanda
dc.contributor.authorTorres Ramirez, Inés de
dc.contributor.authorSantos-Briones, Ignacio
dc.contributor.authorAzpiroz Vidaur, Fernando
dc.contributor.authorLobo Alvarez, Beatriz
dc.contributor.authorAlonso Cotoner, Carmen
dc.contributor.authorSantos Vicente, Fco Javier
dc.contributor.authorGonzalez Castro, Ana Maria
dc.contributor.authorVicario Perez, Maria
dc.date.accessioned2022-09-14T10:13:44Z
dc.date.available2022-09-14T10:13:44Z
dc.date.issued2022-06-28
dc.identifier.citationPardo-Camacho C, Ganda Mall JP, Martínez C, Pigrau M, Expósito E, Albert-Bayo M, et al. Mucosal Plasma Cell Activation and Proximity to Nerve Fibres Are Associated with Glycocalyx Reduction in Diarrhoea-Predominant Irritable Bowel Syndrome: Jejunal Barrier Alterations Underlying Clinical Manifestations. Cells. 2022 Jun 28;11(13):2046.
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/11351/8192
dc.descriptionIntestinal barrier dysfunction; Intestinal glycocalyx; Mucosal nerve fibres
dc.description.abstractIrritable bowel syndrome (IBS) is a disorder of brain-gut interaction characterised by abdominal pain and changes in bowel habits. In the diarrhoea subtype (IBS-D), altered epithelial barrier and mucosal immune activation are associated with clinical manifestations. We aimed to further evaluate plasma cells and epithelial integrity to gain understanding of IBS-D pathophysiology. One mucosal jejunal biopsy and one stool sample were obtained from healthy controls and IBS-D patients. Gastrointestinal symptoms, stress, and depression scores were recorded. In the jejunal mucosa, RNAseq and gene set enrichment analyses were performed. A morphometric analysis by electron microscopy quantified plasma cell activation and proximity to enteric nerves and glycocalyx thickness. Immunoglobulins concentration was assessed in the stool. IBS-D patients showed differential expression of humoral pathways compared to controls. Activation and proximity of plasma cells to nerves and IgG concentration were also higher in IBS-D. Glycocalyx thickness was lower in IBS-D compared to controls, and this reduction correlated with plasma cell activation, proximity to nerves, and clinical symptoms. These results support humoral activity and loss of epithelial integrity as important contributors to gut dysfunction and clinical manifestations in IBS-D. Additional studies are needed to identify the triggers of these alterations to better define IBS-D pathophysiology.
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesCells;11(13)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectCòlon irritable - Complicacions
dc.subjectDiarrea
dc.subjectLeucòcits - Metabolisme
dc.subject.meshIrritable Bowel Syndrome
dc.subject.meshPlasma Cells
dc.subject.meshDiarrhea
dc.titleMucosal Plasma Cell Activation and Proximity to Nerve Fibres Are Associated with Glycocalyx Reduction in Diarrhoea-Predominant Irritable Bowel Syndrome: Jejunal Barrier Alterations Underlying Clinical Manifestations
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/cells11132046
dc.subject.decssíndrome del colon irritable
dc.subject.decscélulas plasmáticas
dc.subject.decsdiarrea
dc.relation.publishversionhttps://doi.org/10.3390/cells11132046
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Pardo-Camacho C] Laboratori d’Immunologia Translacional, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Ganda Mall JP] Laboratori d’Immunologia Translacional, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden. [Martínez C] Vascular and Renal Translational Research Group, Lleida Institute for Biomedical Research Dr. Pifarré. Foundation (IRBLleida), Lleida, Spain. [Pigrau M] Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Expósito E, González-Castro AM] Laboratori d’Immunologia Translacional, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Albert-Bayo M, Melón-Ardanaz E, Fortea M] Laboratori d’Immunologia Translacional, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Nieto A] Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rodiño-Janeiro B] Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Guagnozzi D] Laboratori d’Immunologia Translacional, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Rodriguez-Urrutia A] Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Servei de Salut Mental, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Instituto de Salud Carlos III, Madrid, Spain. [Torres I] Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Servei de Patologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Santos-Briones I] Facultat Ciències de la Salut, Universitat Ramon LLull-Blanquerna, Barcelona, Spain. [Azpiroz F] Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Madrid, Spain. [Lobo B] Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Alonso-Cotoner C, Santos J] Laboratori de Neuroimmuno-Gastroenterologia, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. Servei de Gastroenterologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, Madrid, Spain. [Vicario M] Laboratori d’Immunologia Translacional, Unitat de Recerca d’Aparell Digestiu, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Department of Gastrointestinal Health, Nestlé Institute of Health Sciences, Société des Produits Nestlé S.A., Nestlé Research, Vers-chez-les-Blanc, Lausanne, Switzerland
dc.identifier.pmid35805133
dc.identifier.wos000823994800001
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2017-2020/PI19%2F01643
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2013-2016/PI17%2F01443
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2013-2016/PI15%2F00301
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2013-2016/CPII16%2F00031
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2013-2016/CB06%2F04%2F0021
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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