| dc.contributor | Vall d'Hebron Barcelona Hospital Campus |
| dc.contributor.author | Suescún-Carrero, Sandra Helena |
| dc.contributor.author | Tadger, Philippe |
| dc.contributor.author | Sandoval-Cuellar, Carolina |
| dc.contributor.author | Armadans, Lluis |
| dc.contributor.author | Ramirez Lopez, Laura Ximena |
| dc.date.accessioned | 2022-11-22T12:06:38Z |
| dc.date.available | 2022-11-22T12:06:38Z |
| dc.date.issued | 2022-10-18 |
| dc.identifier.citation | Suescún-Carrero SH, Tadger P, Sandoval Cuellar C, Armadans-Gil L, Ramírez López LX. Rapid diagnostic tests and ELISA for diagnosing chronic Chagas disease: Systematic revision and meta-analysis. PLoS Negl Trop Dis. 2022 Oct 18;16(10):e0010860. |
| dc.identifier.issn | 1935-2735 |
| dc.identifier.uri | https://hdl.handle.net/11351/8519 |
| dc.description | Medical risk factors; Diagnostic medicine; Trypanosoma cruzi |
| dc.description.abstract | Objective
To determine the diagnostic validity of the enzyme-linked immunosorbent assay (ELISA) and Rapid Diagnostic Tests (RDT) among individuals with suspected chronic Chagas Disease (CD).
Methodology
A search was made for studies with ELISA and RDT assays validity estimates as eligibility criteria, published between 2010 and 2020 on PubMed, Web of Science, Scopus, and LILACS. This way, we extracted the data and assessed the risk of bias and applicability of the studies using the QUADAS-2 tool. The bivariate random effects model was also used to estimate the overall sensitivity and specificity through forest-plots, ROC space, and we visually assessed the heterogeneity between studies. Meta-regressions were made using subgroup analysis. We used Deeks’ test to assess the risk of publication bias.
Results
43 studies were included; 27 assessed ELISA tests; 14 assessed RDTs; and 2 assessed ELISA and RDTs, against different reference standards. 51.2 % of them used a non-comparative observational design, and 46.5 % a comparative clinical design (“case-control” type). High risk of bias was detected for patient screening and reference standard. The ELISA tests had a sensitivity of 99% (95% CI: 98–99) and a specificity of 98% (95% CI: 97–99); whereas the Rapid Diagnostic Tests (RDT) had values of 95% (95% CI: 94–97) and 97% (95% CI: 96–98), respectively. Deeks’ test showed asymmetry on the ELISA assays.
Conclusions
ELISA and RDT tests have high validity for diagnosing chronic Chagas disease. The analysis of these two types of evidence in this systematic review and meta-analysis constitutes an input for their use. The limitations included the difficulty in extracting data due to the lack of information in the articles, and the comparative clinical-type design of some studies. |
| dc.language.iso | eng |
| dc.publisher | Public Library of Science |
| dc.relation.ispartofseries | PLoS Neglected Tropical Diseases;16(10) |
| dc.rights | Attribution 4.0 International |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
| dc.source | Scientia |
| dc.subject | Chagas, Malaltia de - Diagnòstic |
| dc.subject.mesh | Chagas Disease |
| dc.subject.mesh | /diagnosis |
| dc.subject.mesh | Diagnostic Tests, Routine |
| dc.title | Rapid diagnostic tests and ELISA for diagnosing chronic Chagas disease: Systematic revision and meta-analysis |
| dc.type | info:eu-repo/semantics/article |
| dc.identifier.doi | 10.1371/journal.pntd.0010860 |
| dc.subject.decs | enfermedad de Chagas |
| dc.subject.decs | /diagnóstico |
| dc.subject.decs | pruebas diagnósticas rutinarias |
| dc.relation.publishversion | https://doi.org/10.1371/journal.pntd.0010860 |
| dc.type.version | info:eu-repo/semantics/publishedVersion |
| dc.audience | Professionals |
| dc.contributor.organismes | Institut Català de la Salut |
| dc.contributor.authoraffiliation | [Suescún-Carrero SH, Sandoval Cuellar C, Ramírez López LX] Universidad de Boyacá, Tunja, Colombia. [Tadger P] Universidad de Boyacá, Tunja, Colombia. Real World Solutions, IQVIA, Zaventem, Belgium. [Armadans-Gil L] Servei de Medicina Preventiva i Epidemiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain |
| dc.identifier.pmid | 36256676 |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess |