Current procedures for the assessment of chronic wound infection are time-consuming and require complex instruments and trained personnel. The incidence of chronic wounds worldwide, and the associated economic burden, urge for simple and cheap point-of-care testing (PoCT) devices for fast on-site diagnosis to enable appropriate early treatment. The enzyme myeloperoxidase (MPO), whose activity in infected wounds is about ten times higher than in non-infected wounds, appears to be a suitable biomarker for wound infection diagnosis. Herein, we develop a single-component foldable paper-based device for the detection of MPO in wound fluids. The analyte detection is achieved in two steps: (i) selective immunocapture of MPO, and (ii) reaction of a specific dye with the captured MPO, yielding a purple color with increasing intensity as a function of the MPO activity in infected wounds in the range of 20-85 U/mL. Ex vivo experiments with wound fluids validated the analytic efficiency of the paper-based device, and the results strongly correlate with a spectrophotometric assay.
Chronic wounds; Infection biomarker; Myeloperoxidase; Colorimetry
Hoyo J, Bassegoda A, Ferreres G, Hinojosa-Caballero D, Gutiérrez-Capitán M, Baldi A, et al. Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device. Int J Mol Sci. 2022 Aug 15;23(16):9129.
Use this identifier for quote and/or link this documenthttps://hdl.handle.net/11351/9583
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