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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorSolé Marce, Cristina
dc.contributor.authorMoline Marimon, Teresa
dc.contributor.authorVidal Borrego, Marta
dc.contributor.authorOrdi Ros, José
dc.contributor.authorCortés Hernandez, Josefina
dc.date.accessioned2020-02-07T08:26:30Z
dc.date.available2020-02-07T08:26:30Z
dc.date.issued2019-07-25
dc.identifier.citationSolé C, Moliné T, Vidal M, Ordi-Ros J, Cortés-Hernández J. An exosomal urinary miRNA signature for early diagnosis of renal fibrosis in lupus nephritis. Cells. 2019 Jul 25;8(8):773.
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/11351/4595
dc.descriptionLupus nephritis; Urinary exosomes; Renal fibrosis
dc.description.abstractFor lupus nephritis (LN) management, it is very important to detect fibrosis at an early stage. Urinary exosomal miRNAs profiling can be used as a potential multi-marker phenotyping tool to identify early fibrosis. We isolated and characterised urinary exosomes and cellular pellets from patients with biopsy-proven LN (n = 45) and healthy controls (n = 20). LN chronicity index (CI) correlated with urinary exosomal miR-21, miR-150, and miR-29c (r = 0.565, 0.840, -0.559,respectively). This miRNA profile distinguished low CI from moderate-high CI in LN patients with a high sensitivity and specificity (94.4% and 99.8%). Furthermore, this multimarker panel predicted an increased risk of progression to end-stage renal disease (ESRD). Pathway analysis identified VEGFA and SP1 as common target genes for the three miRNAs. Immunohistochemistry in LN renal biopsies revealed a significant increase of COL1A1 and COL4A1 correlated with renal chronicity. SP1 decreased significantly in the high-CI group (p = 0.002). VEGFA levels showed no di_erences. In vitro experiments suggest that these miRNA combinations promote renal fibrosis by increasing profibrotic molecules through SP1 and Smad3/TGF_ pathways. In conclusion, a urinary exosomal multimarker panel composed of miR-21, miR-150, and miR-29c provides a non-invasive method to detect early renal fibrosis and predict disease progression in LN
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofseriesCells;8(8)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceScientia
dc.subjectMicroARN
dc.subjectFibrosi
dc.subjectLupus eritematós sistèmic
dc.subject.meshLupus Nephritis
dc.subject.meshCirculating MicroRNA
dc.subject.meshFibrosis
dc.titleAn exosomal urinary miRNA signature for early diagnosis of renal fibrosis in lupus nephritis
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/cells8080773
dc.subject.decsnefritis lúpica
dc.subject.decsmicroARN circulante
dc.subject.decsfibrosis
dc.relation.publishversionhttps://www.mdpi.com/2073-4409/8/8/773
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.authoraffiliation[Solé C, Ordi-Ros J, Cortés-Hernández J] Hospital Universitari Vall d’Hebron, Barcelona, Spain. Unitat de Recerca en Lupus, Vall d'Hebron Institut de Recerca, Barcelona, Spain. [Moliné T, Vidal M] Servei de Patologia Renal, Hospital Universitari Vall d’Hebron, Barcelona, Spain.
dc.identifier.pmid31349698
dc.identifier.wos000484537500006
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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