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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorGregori Font, Josep
dc.contributor.authorCortese, Maria Francesca
dc.contributor.authorPiñana Moro, Maria
dc.contributor.authorCampos Martinez, Carolina
dc.contributor.authorGarcia Cehic, Damir
dc.contributor.authorAndrés Verges, Cristina
dc.contributor.authorCodina Grau, Gema
dc.contributor.authorRando Segura, Ariadna
dc.contributor.authorEsperalba Esquerra, Juliana
dc.contributor.authorSulleiro Igual, Elena
dc.contributor.authorJoseph Munné, Joan
dc.contributor.authorSaubí Roca, Narcís
dc.contributor.authorColomer Castell, Sergi
dc.contributor.authorMartin Perez, Maria Carmen
dc.contributor.authorCastillo Oliveda, Carla
dc.contributor.authorEsteban Mur, Juan Ignacio
dc.contributor.authorPumarola Suñé, Tomàs
dc.contributor.authorRodríguez Frias, Francisco
dc.contributor.authorAntón Pagarolas, Andres
dc.contributor.authorQuer Sivila, Josep
dc.date.accessioned2022-04-22T13:19:54Z
dc.date.available2022-04-22T13:19:54Z
dc.date.issued2021-09-05
dc.identifier.citationGregori J, Cortese MF, Piñana M, Campos C, Garcia-Cehic D, Andrés C, et al. Host-dependent editing of SARS-CoV-2 in COVID-19 patients. Emerg Microbes Infect. 2021;10(1):1777–89.
dc.identifier.issn2222-1751
dc.identifier.urihttp://hdl.handle.net/11351/7379
dc.descriptionSARS-CoV-2; Mutations; Quasispecies
dc.description.abstractA common trait among RNA viruses is their high capability to acquire genetic variability due to viral and host mechanisms. Next-generation sequencing (NGS) analysis enables the deep study of the viral quasispecies in samples from infected individuals. In this study, the viral quasispecies complexity and single nucleotide polymorphisms of the SARS-CoV-2 spike gene of coronavirus disease 2019 (COVID-19) patients with mild or severe disease were investigated using next-generation sequencing (Illumina platform). SARS-CoV-2 spike variability was higher in patients with long-lasting infection. Most substitutions found were present at frequencies lower than 1%, and had an A → G or T → C pattern, consistent with variants caused by adenosine deaminase acting on RNA-1 (ADAR1). ADAR1 affected a small fraction of replicating genomes, but produced multiple, mainly non-synonymous mutations. ADAR1 editing during replication rather than the RNA-dependent RNA polymerase (nsp12) was the predominant mechanism generating SARS-CoV-2 genetic variability. However, the mutations produced are not fixed in the infected human population, suggesting that ADAR1 may have an antiviral role, whereas nsp12-induced mutations occurring in patients with high viremia and persistent infection are the main source of new SARS-CoV-2 variants.
dc.language.isoeng
dc.publisherTaylor & Francis
dc.relation.ispartofseriesEmerging Microbes and Infections;10(1)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectCOVID-19 (Malaltia)
dc.subjectSeqüència d'aminoàcids
dc.subjectVirologia
dc.subject.meshCoronavirus Infections
dc.subject.mesh/virology
dc.subject.meshAmino Acid Sequence
dc.titleHost-dependent editing of SARS-CoV-2 in COVID-19 patients
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1080/22221751.2021.1969868
dc.subject.decsinfecciones por Coronavirus
dc.subject.decs/virología
dc.subject.decssecuencia de aminoácidos
dc.relation.publishversionhttps://doi.org/10.1080/22221751.2021.1969868
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Gregori J] Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Malalties Hepàtiques-Hepatitis Viral, Unitat del Fetge, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain. Roche Diagnostics SL, Barcelona, Spain. [Cortese MF, Saubí N] Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Piñana M, Andrés C, Martin MC, Castillo C] Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Unitat de Virus Respiratoris, Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Campos C, Garcia-Cehic D, Quer J] Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Malalties Hepàtiques-Hepatitis Viral, Unitat del Fetge, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain. [Codina MG, Rando A, Esperalba J, Sulleiro E, Joseph J] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Colomer-Castell S, Esteban JI] Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Malalties Hepàtiques-Hepatitis Viral, Unitat del Fetge, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Pumarola T] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Rodriguez-Frias F ] Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei de Bioquímica i Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Antón A] Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Unitat de Virus Respiratoris, Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain
dc.identifier.pmid34402744
dc.identifier.wos000754901600001
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2013-2016/RD16%2F0016%2F0003
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/PE2017-2020/PI19%2F00301
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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