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dc.contributorVall d'Hebron Barcelona Hospital Campus
dc.contributor.authorTarrés Freixas, Ferran
dc.contributor.authorTrinité, Benjamin
dc.contributor.authorPons-Grifols, Anna
dc.contributor.authorRomero-Durana, Miguel
dc.contributor.authorRiveira Muñoz, Eva
dc.contributor.authorÁvila-Nieto, Carlos
dc.contributor.authorAndrés Verges, Cristina
dc.contributor.authorAntón Pagarolas, Andres
dc.contributor.authorPumarola Suñé, Tomàs
dc.date.accessioned2022-09-07T12:04:11Z
dc.date.available2022-09-07T12:04:11Z
dc.date.issued2022-05
dc.identifier.citationTarrés-Freixas F, Trinité B, Pons-Grífols A, Romero-Durana M, Riveira-Muñoz E, Ávila-Nieto C, et al. Heterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice. Front Microbiol. 2022 May;13:840757.
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11351/8054
dc.descriptionSARS-CoV-2; Viral load; Wildtype mice
dc.description.abstractThe emerging SARS-CoV-2 variants of concern (VOCs) may display enhanced transmissibility, more severity and/or immune evasion; however, the pathogenesis of these new VOCs in experimental SARS-CoV-2 models or the potential infection of other animal species is not completely understood. Here we infected K18-hACE2 transgenic mice with B.1, B.1.351/Beta, B.1.617.2/Delta and BA.1.1/Omicron isolates and demonstrated heterogeneous infectivity and pathogenesis. B.1.351/Beta variant was the most pathogenic, while BA.1.1/Omicron led to lower viral RNA in the absence of major visible clinical signs. In parallel, we infected wildtype (WT) mice and confirmed that, contrary to B.1 and B.1.617.2/Delta, B.1.351/Beta and BA.1.1/Omicron can infect them. Infection in WT mice coursed without major clinical signs and viral RNA was transient and undetectable in the lungs by day 7 post-infection. In silico modeling supported these findings by predicting B.1.351/Beta receptor binding domain (RBD) mutations result in an increased affinity for both human and murine ACE2 receptors, while BA.1/Omicron RBD mutations only show increased affinity for murine ACE2.
dc.language.isoeng
dc.publisherFrontiers Media
dc.relation.ispartofseriesFrontiers in Microbiology;13
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientia
dc.subjectCOVID-19 (Malaltia)
dc.subjectRatolins transgènics
dc.subjectSimulació (Medicina)
dc.subject.meshMice, Transgenic
dc.subject.meshCoronavirus Infections
dc.subject.meshComputer Simulation
dc.titleHeterogeneous Infectivity and Pathogenesis of SARS-CoV-2 Variants Beta, Delta and Omicron in Transgenic K18-hACE2 and Wildtype Mice
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3389/fmicb.2022.840757
dc.subject.decsratones transgénicos
dc.subject.decsinfecciones por Coronavirus
dc.subject.decssimulación por ordenador
dc.relation.publishversionhttps://doi.org/10.3389/fmicb.2022.840757
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.audienceProfessionals
dc.contributor.organismesInstitut Català de la Salut
dc.contributor.authoraffiliation[Tarrés-Freixas F, Trinité B, Pons-Grífols A, Riveira-Muñoz E, Ávila-Nieto C] IrsiCaixa AIDS Research Institute, Can Ruti Campus, UAB, Badalona, Spain. [Romero-Durana M] Barcelona Supercomputing Center, Barcelona, Spain. [Andrés C, Antón A, Pumarola T] Unitat de Virus Respiratoris, Servei de Microbiologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain
dc.identifier.pmid35602059
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess


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